Cell Death Antibodies 2
Anti-EVA1A Antibody (CAB8070)
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- SKU:
- CAB8070
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Description
| Antibody Name: | EVA1A Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8070 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 58-152 of human EVA1A (NP_001128504.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 58-152 of human EVA1A (NP_001128504.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SCHT DCRR RPGK KFLQ DRES SSDS SDSE DGSE DTVS DLSV RRHR RFER TLNK NVFT SAEE LERA QRLE ERER IIRE IWMN GQPE VPGT RSLN RYY |
| Gene ID: | 84141 |
| Uniprot: | Q9H8M9 |
| Cellular Location: | Endoplasmic reticulum membrane, Lysosome membrane, Single-pass membrane protein |
| Calculated MW: | 17kDa |
| Observed MW: | 17KDa |
| Synonyms: | EVA1A, FAM176A, TMEM166 |
| Background: |
| UniProt Protein Function: | TMEM166: Acts as a regulator of programmed cell death, mediating both autophagy and apoptosis. Belongs to the FAM176 family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 2p12 Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; lysosomal membrane; integral to membrane; plasma membrane Biological Process: apoptosis; autophagy |
| UniProt Code: | Q9H8M9 |
| NCBI GenInfo Identifier: | 205277349 |
| NCBI Gene ID: | 84141 |
| NCBI Accession: | NP_115557 |
| UniProt Secondary Accession: | Q9H8M9,Q9HC41, D6W5J3, |
| UniProt Related Accession: | Q9H8M9 |
| Molecular Weight: | SDS-PAGE MW: 17KD |
| NCBI Full Name: | protein eva-1 homolog A |
| NCBI Synonym Full Names: | eva-1 homolog A (C. elegans) |
| NCBI Official Symbol: | EVA1A |
| NCBI Official Synonym Symbols: | FAM176A; TMEM166 |
| NCBI Protein Information: | protein eva-1 homolog A |
| UniProt Protein Name: | Protein eva-1 homolog A |
| UniProt Synonym Protein Names: | Protein FAM176A; Transmembrane protein 166 |
| Protein Family: | Protein |
| UniProt Gene Name: | EVA1A |
| UniProt Entry Name: | EVA1A_HUMAN |
 | Western blot analysis of extracts of 293T cells, using EVA1A antibody (CAB8070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using EVA1A Rabbit pAb (CAB8070) at dilution of 1:100 (40x lens). |
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