Epigenetics & Nuclear Signaling Antibodies 4

Anti-ETV1 Antibody (CAB7184)

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SKU:
CAB7184
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-ETV1 Antibody
Antibody SKU:CAB7184
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ETV1 (NP_004947.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, 22Rv1, HeLa, Mouse testis, Mouse brain, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ETV1 (NP_004947.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDGF YDQQ VPYM VTNS QRGR NCNE KPTN VRKR KFIN RDLA HDSE ELFQ DLSQ LQET WLAE AQVP DNDE QFVP DYQA ESLA FHGL PLKI KKEP HSPC SEIS SACS QEQP FKFS YGEK CLYN VSAY DQKP QVGM RPSN PPTP SSTP VSPL HHAS PNST HTPK PDRA FPAH LPPS QSIP DSSY PMDH RFRR QLSE PCNS FPPL
Gene ID:2115
Uniprot:P50549
Cellular Location:Nucleus
Calculated MW:43kDa/48kDa/50kDa/52kDa/53kDa/55kDa
Observed MW:55kDa
Synonyms:ETV1, ER81
Background:This gene encodes a member of the ETS (E twenty-six) family of transcription factors. The ETS proteins regulate many target genes that modulate biological processes like cell growth, angiogenesis, migration, proliferation and differentiation. All ETS proteins contain an ETS DNA-binding domain that binds to DNA sequences containing the consensus 5'-CGGA[AT]-3'. The protein encoded by this gene contains a conserved short acidic transactivation domain (TAD) in the N-terminal region, in addition to the ETS DNA-binding domain in the C-terminal region. This gene is involved in chromosomal translocations, which result in multiple fusion proteins including EWS-ETV1 in Ewing sarcoma and at least 10 ETV1 partners (see PMID: 19657377, Table 1) in prostate cancer. In addition to chromosomal rearrangement, this gene is overexpressed in prostate cancer, melanoma and gastrointestinal stromal tumor. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
UniProt Protein Function:ETV1: Transcriptional activator that binds to DNA sequences containing the consensus pentanucleotide 5'-CGGA[AT]-3'. Defects in ETV1 are a cause of Ewing sarcoma (ES). A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. A chromosomal aberration involving ETV1 is found in patients with Erwing sarcoma. Translocation t(7;22)(p22;q12) with EWSR1. Belongs to the ETS family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 7p21.3

Cellular Component: nucleus

Molecular Function:protein binding; transcription factor activity

Biological Process: peripheral nervous system neuron development; positive regulation of transcription from RNA polymerase II promoter

NCBI Summary:This gene encodes a member of the ETS (E twenty-six) family of transcription factors. The ETS proteins regulate many target genes that modulate biological processes like cell growth, angiogenesis, migration, proliferation and differentiation. All ETS proteins contain an ETS DNA-binding domain that binds to DNA sequences containing the consensus 5'-CGGA[AT]-3'. The protein encoded by this gene contains a conserved short acidic transactivation domain (TAD) in the N-terminal region, in addition to the ETS DNA-binding domain in the C-terminal region. This gene is involved in chromosomal translocations, which result in multiple fusion proteins including EWS-ETV1 in Ewing sarcoma and at least 10 ETV1 partners (see PMID: 19657377, Table 1) in prostate cancer. In addition to chromosomal rearrangement, this gene is overexpressed in prostate cancer, melanoma and gastrointestinal stromal tumor. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2016]
UniProt Code:P50549
NCBI GenInfo Identifier:12643411
NCBI Gene ID:2115
NCBI Accession:P50549.2
UniProt Secondary Accession:P50549,O75849, Q4KMQ6, Q59GA7, A4D118, B2R768, B7Z2I4 B7Z618, B7Z9P2, C9JT37, E9PHB1, F5GXR2,
UniProt Related Accession:P50549
Molecular Weight:50,217 Da
NCBI Full Name:ETS translocation variant 1
NCBI Synonym Full Names:ETS variant 1
NCBI Official Symbol:ETV1  
NCBI Official Synonym Symbols:ER81  
NCBI Protein Information:ETS translocation variant 1
UniProt Protein Name:ETS translocation variant 1
UniProt Synonym Protein Names:Ets-related protein 81
Protein Family:ETS translocation
UniProt Gene Name:ETV1  
UniProt Entry Name:ETV1_HUMAN
Anti-ETV1 Antibody (CAB7184)Western blot analysis of extracts of various cell lines, using ETV1 antibody (CAB7184) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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