Metabolism Antibodies 1

Anti-ETNK2 Antibody (CAB14628)

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SKU:
CAB14628
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-ETNK2 Antibody
Antibody SKU:CAB14628
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-386 of human ETNK2 (NP_060678.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:293T, DU145, HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-386 of human ETNK2 (NP_060678.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAVP PSAP QPRA SFHL RRHT PCPQ CSWG MEEK AAAS ASCR EPPG PPRA AAVA YFGI SVDP DDIL PGAL RLIQ ELRP HWKP EQVR TKRF TDGI TNKL VACY VEED MQDC VLVR VYGE RTEL LVDR ENEV RNFQ LLRA HSCA PKLY CTFQ NGLC YEYM QGVA LEPE HIRE PRLF RLIA LEMA KIHT IHAN GSLP KPIL WHKM HNYF TLVK NEIN PSLS ADVP KVEV LERE LAWL KEHL SQLE SPVV FCHN DLLC KNII YDSI KGHV RFID YEYA GYNY QAFD IGNH FNEF AGVN EVDY CLYP ARET QLQW LHYY LQAQ KGMA VTPR EVQR LYVQ VNKF ALAS HFFW ALWA LIQN QYST IDFD FLRY AVIR FNQY FKVK PQAS ALEM PK
Gene ID:55224
Uniprot:Q9NVF9
Cellular Location:
Calculated MW:40kDa/44kDa
Observed MW:45kDa
Synonyms:ETNK2, EKI2, HMFT1716
Background:The protein encoded by this gene is a member of choline/ethanolamine kinase family which catalyzes the first step of phosphatidylethanolamine (PtdEtn) biosynthesis via the cytidine diphosphate (CDP) ethanolamine pathway. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:ETNK2: Highly specific for ethanolamine phosphorylation. Does not have choline kinase activity. Belongs to the choline/ethanolamine kinase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Lipid Metabolism - glycerophospholipid; Kinase, other; EC 2.7.1.82

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: cytosol

Molecular Function:ethanolamine kinase activity

Biological Process: phosphatidylethanolamine biosynthetic process

NCBI Summary:The protein encoded by this gene is a member of choline/ethanolamine kinase family which catalyzes the first step of phosphatidylethanolamine (PtdEtn) biosynthesis via the cytidine diphosphate (CDP) ethanolamine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Code:Q9NVF9
NCBI GenInfo Identifier:296439366
NCBI Gene ID:55224
NCBI Accession:Q9NVF9.3
UniProt Secondary Accession:Q9NVF9,Q5SXX5, Q68CK3, Q96G05, B7Z7K1,
UniProt Related Accession:Q9NVF9
Molecular Weight:40,009 Da
NCBI Full Name:Ethanolamine kinase 2
NCBI Synonym Full Names:ethanolamine kinase 2
NCBI Official Symbol:ETNK2  
NCBI Official Synonym Symbols:EKI2; HMFT1716  
NCBI Protein Information:ethanolamine kinase 2
UniProt Protein Name:Ethanolamine kinase 2
UniProt Synonym Protein Names:Ethanolamine kinase-like protein
Protein Family:Ethanolamine kinase
UniProt Gene Name:ETNK2  
UniProt Entry Name:EKI2_HUMAN
Anti-ETNK2 Antibody (CAB14628)Western blot analysis of extracts of various cell lines, using ETNK2 antibody (CAB14628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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