Metabolism Antibodies 1

Anti-ETNK1 Antibody (CAB13224)

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SKU:
CAB13224
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-ETNK1 Antibody
Antibody SKU:CAB13224
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human ETNK1 (NP_061108.2).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human
Positive Samples:293T, OVCAR3, U-87MG
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human ETNK1 (NP_061108.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SPEV PKLN VTVQ DQEE HRCR EGAL SLLQ HLRP HWDP QEVT LQLF TDGI TNKL IGCY VGNT MEDV VLVR IYGN KTEL LVDR DEEV KSFR VLQA HGCA PQLY CTFN NGLC YEFI QGEA LDPK HVCN PAIF RLIA RQLA KIHA IHAH NGWI PKSN LWLK MGKY FSLI PTGF ADED INKR FLSD IPSS QILQ EEMT WMKE ILSN L
Gene ID:55500
Uniprot:Q9HBU6
Cellular Location:Cytoplasm
Calculated MW:27kDa/50kDa
Observed MW:51kDa
Synonyms:ETNK1, EKI, EKI 1, EKI1, Nbla10396
Background:This gene encodes an ethanolamine kinase, which functions in the first committed step of the phosphatidylethanolamine synthesis pathway. This cytosolic enzyme is specific for ethanolamine and exhibits negligible kinase activity on choline. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
UniProt Protein Function:ETNK1: Highly specific for ethanolamine phosphorylation. May be a rate-controlling step in phosphatidylethanolamine biosynthesis. Belongs to the choline/ethanolamine kinase family.
UniProt Protein Details:

Protein type:EC 2.7.1.82; Lipid Metabolism - glycerophospholipid; Kinase, other

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: cytoplasm; cytosol; membrane; nucleoplasm; plasma membrane

Molecular Function:ethanolamine kinase activity; protein binding

Biological Process: phosphatidylethanolamine biosynthetic process

NCBI Summary:This gene encodes an ethanolamine kinase, which functions in the first committed step of the phosphatidylethanolamine synthesis pathway. This cytosolic enzyme is specific for ethanolamine and exhibits negligible kinase activity on choline. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:Q9HBU6
NCBI GenInfo Identifier:14194724
NCBI Gene ID:55500
NCBI Accession:Q9HBU6.1
UniProt Secondary Accession:Q9HBU6,G5E969,
UniProt Related Accession:Q9HBU6
Molecular Weight:27,995 Da
NCBI Full Name:Ethanolamine kinase 1
NCBI Synonym Full Names:ethanolamine kinase 1
NCBI Official Symbol:ETNK1  
NCBI Official Synonym Symbols:EKI; EKI1; EKI 1; Nbla10396  
NCBI Protein Information:ethanolamine kinase 1
UniProt Protein Name:Ethanolamine kinase 1
Protein Family:Ethanolamine kinase
UniProt Gene Name:ETNK1  
UniProt Entry Name:EKI1_HUMAN
Anti-ETNK1 Antibody (CAB13224)Western blot analysis of extracts of various cell lines, using ETNK1 Antibody (CAB13224) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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