Epigenetics & Nuclear Signaling Antibodies 3

Anti-Estrogen Receptor beta Antibody (CAB2546)

(No reviews yet) Write a Review
SKU:
CAB2546
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Estrogen Receptor beta Antibody
Antibody SKU:CAB2546
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Estrogen Receptor beta (NP_001201832.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse testis, Rat testis, Rat ovary
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Estrogen Receptor beta (NP_001201832.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDIK NSPS SLNS PSSY NCSQ SILP LEHG SIYI PSSY VDSH HEYP AMTF YSPA VMNY SIPS NVTN LEGG PGRQ TTSP NVLW PTPG HLSP LVVH RQLS HLYA EPQK SPWC EARS LEHT LPVN RETL KRKV SGNR CASP VTGP GSKR DAHF CAVC SDYA SGYH YGVW SCEG CKAF FKRS IQGH NDYI CPAT NQCT IDKN RRKS CQAC RLRK CYEV GMVK CGSR RERC GYRL VRRQ RSAD EQLH CAGK AKRS GGHA PRVR ELLL DALS PEQL VLTL LEAE PPHV
Gene ID:2100
Uniprot:Q92731
Cellular Location:Nucleus
Calculated MW:35-59kDa
Observed MW:59KDa
Synonyms:ESR2, ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2
Background:This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized.
UniProt Protein Function:ER-beta: a nuclear hormone receptor and transcription factor. Binds and activated by estrogen. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Eight alternatively-spliced isoforms have been described. Isoform beta-cx lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.
UniProt Protein Details:

Protein type:DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 14q23.2

Cellular Component: nucleoplasm; mitochondrion; extracellular region; nucleus

Molecular Function:receptor antagonist activity; protein binding; ligand-dependent nuclear receptor activity; estrogen receptor activity; enzyme binding; DNA binding; zinc ion binding; transcription coactivator activity; estrogen response element binding; steroid hormone receptor activity; transcription factor activity; steroid binding

Biological Process: transcription initiation from RNA polymerase II promoter; estrogen receptor signaling pathway; induction of apoptosis by hormones; neuron migration; vagina development; negative regulation of transcription from RNA polymerase II promoter; uterus development; signal transduction; cell-cell signaling; ovarian follicle development; regulation of transcription, DNA-dependent; steroid hormone mediated signaling; gene expression; positive regulation of transcription factor activity; negative regulation of cell growth; brain development; negative regulation of epithelial cell proliferation

NCBI Summary:This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]
UniProt Code:Q92731
NCBI GenInfo Identifier:6166154
NCBI Gene ID:2100
NCBI Accession:Q92731.2
UniProt Related Accession:Q92731
Molecular Weight:Predicted: 53 kDa
NCBI Full Name:Estrogen receptor beta
NCBI Synonym Full Names:estrogen receptor 2 (ER beta)
NCBI Official Symbol:ESR2  
NCBI Official Synonym Symbols:Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA  
NCBI Protein Information:estrogen receptor beta; estrogen receptor beta 4; nuclear receptor subfamily 3 group A member 2
UniProt Protein Name:5p152
UniProt Synonym Protein Names:5p152
Protein Family:Estrogen receptor
UniProt Gene Name:ESR2  
UniProt Entry Name:Q7LCB3_HUMAN
Anti-Estrogen Receptor beta Antibody (CAB2546)Western blot analysis of extracts of various cell lines, using Estrogen Receptor beta Rabbit pAb (CAB2546) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
View AllClose

0 Reviews

View AllClose

Related Products