Antibody Name:	Anti-ESRRA Antibody
Antibody SKU:	CAB14184
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-423 of human ESRRA (NP_004442.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Mouse kidney, Mouse heart, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-423 of human ESRRA (NP_004442.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSSQ VVGI EPLY IKAE PASP DSPK GSSE TETE PPVA LAPG PAPT RCLP GHKE EEDG EGAG PGEQ GGGK LVLS SLPK RLCL VCGD VASG YHYG VASC EACK AFFK RTIQ GSIE YSCP ASNE CEIT KRRR KACQ ACRF TKCL RVGM LKEG VRLD RVRG GRQK YKRR PEVD PLPF PGPF PAGP LAVA GGPR KTAA PVNA LVSH LLVV EPEK LYAM PDPA GPDG HLPA VATL CDLF DREI VVTI SWAK SIPG FSSL SLSD QMSV LQSV WMEV LVLG VAQR SLPL QDEL AFAE DLVL DEEG ARAA GLGE LGAA LLQL VRRL QALR LERE EYVL LKAL ALAN SDSV HIED AEAV EQLR EALH EALL EYEA GRAG PGGG AERR RAGR LLLT LPLL RQTA GKVL AHFY GVKL EGKV PMHK LFLE MLEA MMD
Gene ID:	2101
Uniprot:	P11474
Cellular Location:	Nucleus
Calculated MW:	45kDa
Observed MW:	46kDa

Synonyms:

ESRRA, ERR1, ERRa, ERRalpha, ESRL1, NR3B1

Background:

The protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. Alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	ERR1: Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. Belongs to the nuclear hormone receptor family. NR3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor Chromosomal Location of Human Ortholog: 11q13 Cellular Component: microtubule cytoskeleton; nucleoplasm; intercellular bridge; nucleolus; nucleus Molecular Function:protein domain specific binding; ligand-dependent nuclear receptor activity; protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; steroid binding Biological Process: transcription initiation from RNA polymerase II promoter; mitochondrion organization and biogenesis; intracellular receptor-mediated signaling pathway; regulation of osteoblast differentiation; organelle organization and biogenesis; negative regulation of transcription from RNA polymerase II promoter; regulation of cell proliferation; regulation of osteoclast differentiation; regulation of transcription, DNA-dependent; cartilage development; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter
NCBI Summary:	The protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]
UniProt Code:	P11474
NCBI GenInfo Identifier:	215274146
NCBI Gene ID:	2101
NCBI Accession:	P11474.3
UniProt Related Accession:	P11474
Molecular Weight:
NCBI Full Name:	Steroid hormone receptor ERR1
NCBI Synonym Full Names:	estrogen related receptor alpha
NCBI Official Symbol:	ESRRA
NCBI Official Synonym Symbols:	ERR1; ERRa; ESRL1; NR3B1; ERRalpha
NCBI Protein Information:	steroid hormone receptor ERR1
UniProt Protein Name:	Steroid hormone receptor ERR1
UniProt Synonym Protein Names:	Estrogen receptor-like 1; Estrogen-related receptor alpha; ERR-alpha; Nuclear receptor subfamily 3 group B member 1
Protein Family:	Enolase-related protein
UniProt Gene Name:	ESRRA
UniProt Entry Name:	ERR1_HUMAN

	Western blot analysis of extracts of various cell lines, using ESRRA antibody (CAB14184) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human uterus using ESRRA antibody (CAB14184) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using ESRRA antibody (CAB14184) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using ESRRA antibody (CAB14184) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of HeLa cells using ESRRA antibody (CAB14184) at dilution of 1:100. Blue: DAPI for nuclear staining.