Antibody Name:	Anti-ESPN Antibody
Antibody SKU:	CAB15908
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 585-854 of human ESPN (NP_113663.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 585-854 of human ESPN (NP_113663.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	CAAD PKAS RELP PPPP PPPP PLPE AASS PPPA PPLP LESA GPGC GQRR SSSS TGST KSFN MMSP TGDN SELL AEIK AGKS LKPT PQSK GLTT VFSG IGQP AFQP DSPL PSVS PALS PVRS PTPP AAGF QPLL NGSL VPVP PTTP APGV QLDV EALI PTHD EQGR PIPE WKRQ VMVR KMQL KMQE EEEQ RRKE EEEE ARLA SMPA WRRD LLRK KLEE EREQ KRKE EERQ KQEE LRRE KEQS EKLR TLGY DESK LAPW QRQV ILKK GDIA KY
Gene ID:	83715
Uniprot:	B1AK53
Cellular Location:	Cell projection, Cytoplasm, cytoskeleton, microvillus, stereocilium
Calculated MW:	31kDa/91kDa
Observed MW:	110-120kDa

Synonyms:

ESPN, DFNB36, LP2654, espin

Background:

This gene encodes a multifunctional actin-bundling protein. It plays a major role in regulating the organization, dimensions, dynamics, and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in various mechanosensory and chemosensory cells. Mutations in this gene are associated with autosomal recessive neurosensory deafness, and autosomal dominant sensorineural deafness without vestibular involvement.

UniProt Protein Function:	ESPN: Multifunctional actin-bundling protein. Plays a major role in regulating the organization, dimensions, dynamics and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in variouS mechanosensory and chemosensory cells. Defects in ESPN are the cause of deafness autosomal recessive type 36 with or without vestibular involvement (DFNB36). A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Actin-binding; Cytoskeletal Chromosomal Location of Human Ortholog: 1p36.31 Cellular Component: brush border; filamentous actin Molecular Function:actin filament binding; SH3 domain binding Disease: Deafness, Autosomal Recessive 36, With Or Without Vestibular Involvement
NCBI Summary:	This gene encodes a multifunctional actin-bundling protein. It plays a major role in regulating the organization, dimensions, dynamics, and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in various mechanosensory and chemosensory cells. Mutations in this gene are associated with autosomal recessive neurosensory deafness, and autosomal dominant sensorineural deafness without vestibular involvement. [provided by RefSeq, Nov 2009]
UniProt Code:	B1AK53
NCBI GenInfo Identifier:	189037868
NCBI Gene ID:	83715
NCBI Accession:	B1AK53.1
UniProt Secondary Accession:	B1AK53,Q6XYB2, Q9H0A2, Q9Y329,
UniProt Related Accession:	B1AK53
Molecular Weight:	31,636 Da
NCBI Full Name:	Espin
NCBI Synonym Full Names:	espin
NCBI Official Symbol:	ESPN
NCBI Official Synonym Symbols:	DFNB36; LP2654
NCBI Protein Information:	espin
UniProt Protein Name:	Espin
UniProt Synonym Protein Names:	Autosomal recessive deafness type 36 protein; Ectoplasmic specialization protein
Protein Family:	Espin
UniProt Gene Name:	ESPN
UniProt Entry Name:	ESPN_HUMAN

	Western blot analysis of extracts of various cell lines, using ESPN antibody (CAB15908) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded Human colon carcinoma using ESPN Rabbit pAb (CAB15908) at dilution of 1:100 (40x lens).