KO Validated Antibodies 2

Anti-ESD Antibody (CAB19863)[KO Validated]

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SKU:
CAB19863
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ESD Antibody [KO Validated]
Antibody SKU:CAB19863
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant protein of human ESD.
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:293T
Immunogen:Recombinant protein of human ESD.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:2098
Uniprot:P10768
Cellular Location:Cytoplasm, Cytoplasmic vesicle
Calculated MW:31kDa
Observed MW:32KDa
Anti-ESD Antibody [KO Validated] (CAB19863)Western blot analysis of extracts from normal (control) and ESD knockout (KO) 293T cells, using ESD antibody (CAB19863) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Synonyms:ESD, FGH
Background:This gene encodes a serine hydrolase that belongs to the esterase D family. The encoded enzyme is active toward numerous substrates including O-acetylated sialic acids, and it may be involved in the recycling of sialic acids. This gene is used as a genetic marker for retinoblastoma and Wilson's disease.
UniProt Protein Function:esterase D: an enzyme that catalyzes the conversion of carboxylic esters and H2O into alcohol and a carboxylic anion.
UniProt Protein Details:

Protein type:EC 3.1.2.12; EC 3.1.1.56; Hydrolase

Chromosomal Location of Human Ortholog: 13q14.1-q14.2

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane

Molecular Function:S-formylglutathione hydrolase activity; methylumbelliferyl-acetate deacetylase activity; hydrolase activity, acting on ester bonds

Biological Process: formaldehyde catabolic process

NCBI Summary:This gene encodes a serine hydrolase that belongs to the esterase D family. The encoded enzyme is active toward numerous substrates including O-acetylated sialic acids, and it may be involved in the recycling of sialic acids. This gene is used as a genetic marker for retinoblastoma and Wilson's disease. [provided by RefSeq, Feb 2009]
UniProt Code:P10768
NCBI GenInfo Identifier:544254
NCBI Gene ID:2098
NCBI Accession:P10768.2
UniProt Secondary Accession:P10768,Q5TBU8, Q5TBV0, Q5TBV2, Q9BVJ2,
UniProt Related Accession:P10768
Molecular Weight:282
NCBI Full Name:S-formylglutathione hydrolase
NCBI Synonym Full Names:esterase D
NCBI Official Symbol:ESD  
NCBI Official Synonym Symbols:FGH  
NCBI Protein Information:S-formylglutathione hydrolase; esterase 10; esterase D/formylglutathione hydrolase; methylumbelliferyl-acetate deacetylase
UniProt Protein Name:S-formylglutathione hydrolase
UniProt Synonym Protein Names:Esterase D; Methylumbelliferyl-acetate deacetylase (EC:3.1.1.56)
UniProt Gene Name:ESD  
UniProt Entry Name:ESTD_HUMAN
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