Name: Anti-ERVW-1 Antibody (CAB16522)
Brand: Developmental Biology
SKU: CAB16522
Availability: OutOfStock

Antibody Name:	Anti-ERVW-1 Antibody
Antibody SKU:	CAB16522
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ERVW-1 (NP_001124397.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	U-251MG, 293T, mouse brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ERVW-1 (NP_001124397.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MALP YHIF LFTV LLPS FTLT APPP CRCM TSSS PYQE FLWR MQRP GNID APSY RSLS KGTP TFTA HTHM PRNC YHSA TLCM HANT HYWT GKMI NPSC PGGL
Gene ID:	30816
Uniprot:	Q9UQF0
Cellular Location:	Cell membrane, Peripheral membrane protein, Single-pass type I membrane protein, Virion
Calculated MW:	59kDa
Observed MW:	55kDa

Synonyms:

ERVW-1, ENV, ENVW, ERVWE1, HERV-7q, HERV-W-ENV, HERV7Q, HERVW, HERVWENV

Background:

Many different human endogenous retrovirus (HERV) families are expressed in normal placental tissue at high levels, suggesting that HERVs are functionally important in reproduction. This gene is part of an HERV provirus on chromosome 7 that has inactivating mutations in the gag and pol genes. This gene is the envelope glycoprotein gene which appears to have been selectively preserved. The gene's protein product is expressed in the placental syncytiotrophoblast and is involved in fusion of the cytotrophoblast cells to form the syncytial layer of the placenta. The protein has the characteristics of a typical retroviral envelope protein, including a furin cleavage site that separates the surface (SU) and transmembrane (TM) proteins which form a heterodimer. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	ERVWE1: Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has retained its original fusogenic properties and participates in trophoblast fusion during placenta morphogenesis. Belongs to the gamma type-C retroviral envelope protein family. HERV class-I W env subfamily.
UniProt Protein Details:	Protein type:Membrane protein, integral; Cell surface Chromosomal Location of Human Ortholog: 7q21.2 Biological Process: anatomical structure morphogenesis; syncytium formation
NCBI Summary:	Many different human endogenous retrovirus (HERV) families are expressed in normal placental tissue at high levels, suggesting that HERVs are functionally important in reproduction. This gene is part of an HERV provirus on chromosome 7 that has inactivating mutations in the gag and pol genes. This gene is the envelope glycoprotein gene which appears to have been selectively preserved. The gene's protein product is expressed in the placental syncytiotrophoblast and is involved in fusion of the cytotrophoblast cells to form the syncytial layer of the placenta. The protein has the characteristics of a typical retroviral envelope protein, including a furin cleavage site that separates the surface (SU) and transmembrane (TM) proteins which form a heterodimer. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]
UniProt Code:	Q9UQF0
NCBI GenInfo Identifier:	47605755
NCBI Gene ID:	30816
NCBI Accession:	Q9UQF0.1
UniProt Secondary Accession:	Q9UQF0,O95244, O95245, Q8NHY7, Q9NRZ2, Q9NZG3, B2RPD4
UniProt Related Accession:	Q9UQF0
Molecular Weight:	59,866 Da
NCBI Full Name:	Syncytin-1
NCBI Synonym Full Names:	endogenous retrovirus group W member 1
NCBI Official Symbol:	ERVW-1
NCBI Official Synonym Symbols:	ENV; ENVW; HERVW; ERVWE1; HERV7Q; HERV-7q; HERVWENV; HERV-W-ENV
NCBI Protein Information:	syncytin-1
UniProt Protein Name:	Syncytin-1
UniProt Synonym Protein Names:	Endogenous retrovirus group W member 1; Env-W; Envelope polyprotein gPr73; Enverin; HERV-7q Envelope protein; HERV-W envelope protein; HERV-W_7q21.2 provirus ancestral Env polyprotein; Syncytin
Protein Family:	Syncytin
UniProt Gene Name:	ERVW-1
UniProt Entry Name:	SYCY1_HUMAN

Western blot analysis of extracts of various cell lines, using ERVW-1 antibody (CAB16522) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)