Signal Transduction Antibodies 2
Anti-ERP27 Antibody (CAB15944)
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- SKU:
- CAB15944
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-ERP27 Antibody |
| Antibody SKU: | CAB15944 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human ERP27 (NP_689534.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse pancreas, Mouse spleen, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human ERP27 (NP_689534.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEAA PSRF MFLL FLLT CELA AEVA AEVE KSSD GPGA AQEP TWLT DVPA AMEF IAAT EVAV IGFF QDLE IPAV PILH SMVQ KFPG VSFG ISTD SEVL THYN ITGN TICL FRLV DNEQ LNLE DEDI ESID ATKL SRFI EINS LHMV TEYN PVTV IGLF NSVI QIHL LLIM NKAS PEYE ENMH RYQK AAKL FQGK ILFI LVDS GMKE NGKV ISFF KLKE SQLP ALAI YQTL DDEW DTLP TAEV SVEH VQNF CDGF LSGK LLKE NRES EGKT PKVE L |
| Gene ID: | 121506 |
| Uniprot: | Q96DN0 |
| Cellular Location: | Endoplasmic reticulum lumen |
| Calculated MW: | 30kDa |
| Observed MW: | 35kDa |
| Synonyms: | ERP27, C12orf46, PDIA8 |
| Background: | This gene encodes a noncatalytic member of the protein disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins. The canonical protein has an N-terminal signal sequence, two thioredoxin (TRX)-like domains and a C-terminal ER-retention sequence. Alternative splicing results in multiple transcript variants encoding distinct isoforms; some of which lack domains present in the canonical protein. |
| UniProt Protein Function: | ERP27: Belongs to the protein disulfide isomerase family. Interacts with PDIA3. Binds somatostatin-14 via hydrophobic interactions |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 12p12.3 Cellular Component: endoplasmic reticulum Molecular Function:protein binding; protein disulfide isomerase activity Biological Process: protein folding |
| NCBI Summary: | ERP27 is a noncatalytic member of the protein disulfide isomerase (PDI; see MIM 608012) family of endoplasmic reticulum (ER) proteins (Alanen et al., 2006 [PubMed 16940051]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q96DN0 |
| NCBI GenInfo Identifier: | 74731474 |
| NCBI Gene ID: | 121506 |
| NCBI Accession: | Q96DN0.1 |
| UniProt Related Accession: | Q96DN0 |
| Molecular Weight: | 30,480 Da |
| NCBI Full Name: | Endoplasmic reticulum resident protein 27 |
| NCBI Synonym Full Names: | endoplasmic reticulum protein 27 |
| NCBI Official Symbol: | ERP27 |
| NCBI Official Synonym Symbols: | PDIA8; C12orf46 |
| NCBI Protein Information: | endoplasmic reticulum resident protein 27 |
| UniProt Protein Name: | Endoplasmic reticulum resident protein 27 |
| Protein Family: | Endoplasmic reticulum resident protein |
| UniProt Gene Name: | ERP27 |
| UniProt Entry Name: | ERP27_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ERP27 antibody (CAB15944) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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