Cell Biology Antibodies 8
Anti-ERO1LB Antibody (CAB3682)
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- SKU:
- CAB3682
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ERO1LB Antibody |
| Antibody SKU: | CAB3682 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 34-230 of human ERO1LB (NP_063944.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-87MG, DU145, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 34-230 of human ERO1LB (NP_063944.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QVTG VLDD CLCD IDSI DNFN TYKI FPKI KKLQ ERDY FRYY KVNL KRPC PFWA EDGH CSIK DCHV EPCP ESKI PVGI KAGH SNKY LKMA NNTK ELED CEQA NKLG AINS TLSN QSKE AFID WARY DDSR DHFC ELDD ERSP AAQY VDLL LNPE RYTG YKGT SAWR VWNS IYEE NCFK PRSV YRPL NPLA PSRG EDDG E |
| Gene ID: | 56605 |
| Uniprot: | Q86YB8 |
| Cellular Location: | Endoplasmic reticulum membrane, Lumenal side, Peripheral membrane protein |
| Calculated MW: | 16kDa/53kDa |
| Observed MW: | 48kDa |
| Synonyms: | ERO1B, ERO1LB, Ero1beta |
| Background: |
| UniProt Protein Function: | ERO1LB: Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the being a source of oxidative stress. Required for the folding of cell, thereby being a source of oxidative stress. Belongs to the EROs family. |
| UniProt Protein Details: | Protein type:Oxidoreductase; EC 1.8.4.-; Endoplasmic reticulum Chromosomal Location of Human Ortholog: 1q42.2-q43 Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum Molecular Function:protein binding; unfolded protein binding; protein disulfide oxidoreductase activity; protein disulfide isomerase activity; oxidoreductase activity; oxidoreductase activity, acting on sulfur group of donors, disulfide as acceptor Biological Process: insulin processing; protein maturation via protein folding; extracellular matrix organization and biogenesis; 4-hydroxyproline metabolic process; protein folding; cell redox homeostasis; glucose homeostasis |
| UniProt Code: | Q86YB8 |
| NCBI GenInfo Identifier: | 116241353 |
| NCBI Gene ID: | 56605 |
| NCBI Accession: | Q86YB8.2 |
| UniProt Secondary Accession: | Q86YB8,Q5T1H4, Q8IZ11, Q9NR62, B4DF57, |
| UniProt Related Accession: | Q86YB8 |
| Molecular Weight: | |
| NCBI Full Name: | ERO1-like protein beta |
| NCBI Synonym Full Names: | ERO1-like beta (S. cerevisiae) |
| NCBI Official Symbol: | ERO1LB |
| NCBI Protein Information: | ERO1-like protein beta; ERO1-L-beta; endoplasmic oxidoreductin-1-like protein B; endoplasmic reticulum oxidoreductin-1-like protein B; oxidoreductin-1-L-beta |
| UniProt Protein Name: | ERO1-like protein beta |
| UniProt Synonym Protein Names: | Endoplasmic reticulum oxidoreductin-1-like protein B; Oxidoreductin-1-L-beta |
| UniProt Gene Name: | ERO1LB |
| UniProt Entry Name: | ERO1B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ERO1LB antibody (CAB3682) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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