Cell Death Antibodies 2
Anti-ERN1 Antibody (CAB17940)
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- SKU:
- CAB17940
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-ERN1 Antibody |
| Antibody SKU: | CAB17940 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human ERN1. |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | Raji |
| Immunogen: | Recombinant protein of human ERN1. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 2081 |
| Uniprot: | O75460 |
| Cellular Location: | Endoplasmic reticulum membrane, Single-pass type I membrane protein |
| Calculated MW: | 6kDa/109kDa |
| Observed MW: | 130kDa |
 | Western blot analysis of extracts of Raji cells, using ERN1 antibody (CAB17940) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
| Synonyms: | ERN1, IRE1, IRE1P, IRE1a, hIRE1p |
| Background: | The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. |
| NCBI Summary: | This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017] |
| UniProt Code: | O75460 |
| NCBI GenInfo Identifier: | 193806335 |
| NCBI Gene ID: | 2081 |
| NCBI Accession: | O75460.2 |
| UniProt Secondary Accession: | O75460,Q76MJ5, |
| UniProt Related Accession: | O75460 |
| Molecular Weight: | Observed: 110 kDaPredicted: 110 kDa |
| NCBI Full Name: | Serine/threonine-protein kinase/endoribonuclease IRE1 |
| NCBI Synonym Full Names: | endoplasmic reticulum to nucleus signaling 1 |
| NCBI Official Symbol: | ERN1 |
| NCBI Official Synonym Symbols: | IRE1; IRE1P; IRE1a; hIRE1p |
| NCBI Protein Information: | serine/threonine-protein kinase/endoribonuclease IRE1 |
| UniProt Protein Name: | Serine/threonine-protein kinase/endoribonuclease IRE1 |
| UniProt Synonym Protein Names: | Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1; hIRE1p; Ire1-alpha; IRE1a |
| Protein Family: | Serine/threonine-protein kinase/endoribonuclease |
| UniProt Gene Name: | ERN1 |
| UniProt Entry Name: | ERN1_HUMAN |
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