Metabolism Antibodies 3

Anti-Erlin-2 Antibody (CAB8314)

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SKU:
CAB8314

Description

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Antibody Name:ERLIN2 Rabbit Polyclonal Antibody
Antibody SKU:CAB8314
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-152 of human ERLIN2 (NP_001003790.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-937, U-251MG
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-152 of human ERLIN2 (NP_001003790.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KIEE GHIG VYYR GGAL LTST SGPG FHLM LPFI TSYK SVQT TLQT DEVK NVPC GTSG GVMI YFDR IEVV NFLV PNAV YDIV KNYT ADYD KALI FNKI HHEL NQFC SVHT LQEV YIEL FGLE NDFS QESS
Gene ID:11160
Uniprot:O94905
Cellular Location:Endoplasmic reticulum membrane, Single-pass type II membrane protein
Calculated MW:16kDa/22kDa/37kDa
Observed MW:38kDa
Synonyms:ERLIN2, C8orf2, Erlin-2, NET32, SPFH2, SPG18, erlin-2
Background:This gene encodes a member of the SPFH domain-containing family of lipid raft-associated proteins. The encoded protein is localized to lipid rafts of the endoplasmic reticulum and plays a critical role in inositol 1, 4, 5-trisphosphate (IP3) signaling by mediating ER-associated degradation of activated IP3 receptors. Mutations in this gene are a cause of spastic paraplegia-18 (SPG18). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
UniProt Protein Function:SPFH2: Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Also involved in ITPR1 degradation by the ERAD pathway. Interacts with activated ITPR1, independently of the degree of ITPR1 polyubiquitination. Forms a heteromeric complex with ERLIN1. Ubiquitous. Belongs to the band 7/mec-2 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: cytoplasm; endoplasmic reticulum; endoplasmic reticulum membrane; lipid raft; plasma membrane; protein complex

Molecular Function:cholesterol binding; protein binding; protein-tyrosine kinase activity; ubiquitin protein ligase binding

Biological Process: ER-associated protein catabolic process; negative regulation of cholesterol biosynthetic process; negative regulation of fatty acid biosynthetic process; SREBP-mediated signaling pathway

Disease: Spastic Paraplegia 18, Autosomal Recessive

NCBI Summary:This gene encodes a member of the SPFH domain-containing family of lipid raft-associated proteins. The encoded protein is localized to lipid rafts of the endoplasmic reticulum and plays a critical role in inositol 1,4,5-trisphosphate (IP3) signaling by mediating ER-associated degradation of activated IP3 receptors. Mutations in this gene are a cause of spastic paraplegia-18 (SPG18). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
UniProt Code:O94905
NCBI GenInfo Identifier:38257366
NCBI Gene ID:11160
NCBI Accession:O94905.1
UniProt Secondary Accession:O94905,Q6NW21, Q86VS6, Q86W49, A0JLQ1, A8K5S9, B4DM38 D3DSW0,
UniProt Related Accession:O94905
Molecular Weight:22,939 Da
NCBI Full Name:Erlin-2
NCBI Synonym Full Names:ER lipid raft associated 2
NCBI Official Symbol:ERLIN2  
NCBI Official Synonym Symbols:NET32; SPFH2; SPG18; C8orf2; Erlin-2  
NCBI Protein Information:erlin-2
UniProt Protein Name:Erlin-2
UniProt Synonym Protein Names:Endoplasmic reticulum lipid raft-associated protein 2; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2; SPFH domain-containing protein 2
Protein Family:Erlin
UniProt Gene Name:ERLIN2  
UniProt Entry Name:ERLN2_HUMAN
Anti-Erlin-2 Polyclonal Antibody (CAB8314)Western blot analysis of extracts of various cell lines, using ERLIN2 antibody (CAB8314) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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Additional Information

Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
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