Antibody Name:	Anti-ERG Antibody
Antibody SKU:	CAB1240
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ERG (NP_891548.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	MCF7, THP-1, HeLa, BT-474, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ERG (NP_891548.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAST IKEA LSVV SEDQ SLFE CAYG TPHL AKTE MTAS SSSD YGQT SKMS PRVP QQDW LSQP PARV TIKM ECNP SQVN GSRN SPDE CSVA KGGK MVGS PDTV GMNY GSYM EEKH MPPP NMTT NERR VIVP ADPT LWST DHVR QWLE WAVK EYGL PDVN ILLF QNID GKEL CKMT KDDF QRLT PSYN ADIL LSHL HYLR ETPL PHLT SDDV DKAL QNSP RLMH ARNT GGAA FIFP NTSV YPEA TQRI TTRP DLPY EPPR RSAW TGHG HPTP QSKA AQPS PSTV
Gene ID:	2078
Uniprot:	P11308
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	35kDa/36kDa/41kDa/52kDa/53kDa/54kDa
Observed MW:	60kDa

Synonyms:

ERG, erg-3, p55

Background:

This gene encodes a member of the erythroblast transformation-specific (ETS) family of transcriptions factors. All members of this family are key regulators of embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. The protein encoded by this gene is mainly expressed in the nucleus. It contains an ETS DNA-binding domain and a PNT (pointed) domain which is implicated in the self-association of chimeric oncoproteins. This protein is required for platelet adhesion to the subendothelium, inducing vascular cell remodeling. It also regulates hematopoesis, and the differentiation and maturation of megakaryocytic cells. This gene is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia. More than two dozens of transcript variants generated from combinatorial usage of three alternative promoters and multiple alternative splicing events have been reported, but the full-length nature of many of these variants has not been determined.

UniProt Protein Function:	ERG: Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure. Defects in ERG are a cause of Ewing sarcoma (ES). A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1. Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4. Belongs to the ETS family. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Oncoprotein; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: cytoplasm; nucleus; ribonucleoprotein complex Molecular Function:signal transducer activity; protein binding; DNA binding; sequence-specific DNA binding Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; cell proliferation; multicellular organismal development; cell differentiation; signal transduction; protein amino acid phosphorylation
NCBI Summary:	This gene encodes a member of the erythroblast transformation-specific (ETS) family of transcriptions factors. All members of this family are key regulators of embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. The protein encoded by this gene is mainly expressed in the nucleus. It contains an ETS DNA-binding domain and a PNT (pointed) domain which is implicated in the self-association of chimeric oncoproteins. This protein is required for platelet adhesion to the subendothelium, inducing vascular cell remodeling. It also regulates hematopoesis, and the differentiation and maturation of megakaryocytic cells. This gene is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia. More than two dozens of transcript variants generated from combinatorial usage of three alternative promoters and multiple alternative splicing events have been reported, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Apr 2014]
UniProt Code:	P11308
NCBI GenInfo Identifier:	152031600
NCBI Gene ID:	2078
NCBI Accession:	P11308.2
UniProt Related Accession:	P11308
Molecular Weight:	57.8
NCBI Full Name:	Transcriptional regulator ERG
NCBI Synonym Full Names:	ETS transcription factor ERG
NCBI Official Symbol:	ERG
NCBI Official Synonym Symbols:	p55; erg-3
NCBI Protein Information:	transcriptional regulator ERG
UniProt Protein Name:	Transcriptional regulator ERG
UniProt Synonym Protein Names:	Transforming protein ERG
Protein Family:	Transcriptional regulator
UniProt Gene Name:	ERG
UniProt Entry Name:	ERG_HUMAN

	Western blot analysis of extracts of various cell lines, using ERG antibody (CAB1240) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of HeLa cells using ERG antibody (CAB1240). Blue: DAPI for nuclear staining.