Epigenetics & Nuclear Signaling Antibodies 4
Anti-ERCC4 Antibody (CAB8119)
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- SKU:
- CAB8119
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IP
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | ERCC4 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8119 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 600-700 of human ERCC4 (NP_005227.1). |
| Application: | WB IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IP 1:20 - 1:50 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, HT-1080 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 600-700 of human ERCC4 (NP_005227.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GKPL RVYF LIYG GSTE EQRY LTAL RKEK EAFE KLIR EKAS MVVP EERE GRDE TNLD LVRG TASA DVST DTRK AGGQ EQNG TQQS IVVD MREF RSEL PSLI H |
| Gene ID: | 2072 |
| Uniprot: | Q92889 |
| Cellular Location: | Nucleus |
| Calculated MW: | 42kDa/104kDa |
| Observed MW: | 120kDa |
| Synonyms: | ERCC4, ERCC11, FANCQ, RAD1, XFEPS, XPF |
| Background: | The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6). |
| UniProt Protein Function: | Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link. |
| NCBI Summary: | The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).[provided by RefSeq, Mar 2009] |
| UniProt Code: | Q92889 |
| NCBI GenInfo Identifier: | 4885217 |
| NCBI Gene ID: | 2072 |
| NCBI Accession: | NP_005227.1 |
| UniProt Secondary Accession: | Q92889,O00140, Q8TD83, A5PKV6, A8K111, |
| UniProt Related Accession: | Q92889 |
| Molecular Weight: | 103 kDa |
| NCBI Full Name: | DNA repair endonuclease XPF |
| NCBI Synonym Full Names: | ERCC excision repair 4, endonuclease catalytic subunit |
| NCBI Official Symbol: | ERCC4 |
| NCBI Official Synonym Symbols: | XPF; RAD1; FANCQ; XFEPS; ERCC11 |
| NCBI Protein Information: | DNA repair endonuclease XPF |
| UniProt Protein Name: | DNA repair endonuclease XPF |
| UniProt Synonym Protein Names: | DNA excision repair protein ERCC-4; DNA repair protein complementing XP-F cells; Xeroderma pigmentosum group F-complementing protein |
| Protein Family: | Positive control factor |
| UniProt Gene Name: | ERCC4 |
 | Western blot analysis of extracts of various cell lines, using ERCC4 antibody (CAB8119) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunoprecipitation analysis of 150ug extracts of 293T cells using 3ug ERCC4 antibody (CAB8119). Western blot was performed from the immunoprecipitate using ERCC4 antibody (CAB8119) at a dilition of 1:1000. |
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