Epigenetics & Nuclear Signaling Antibodies 4

Anti-ERCC4 Antibody (CAB8119)

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SKU:
CAB8119
Product Type:
Antibody
Applications:
WB
Applications:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:ERCC4 Rabbit Polyclonal Antibody
Antibody SKU:CAB8119
Antibody Size:20uL, 50uL, 100uL
Application:WB IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 600-700 of human ERCC4 (NP_005227.1).
Application:WB IP
Recommended Dilution:WB 1:500 - 1:2000 IP 1:20 - 1:50
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, HT-1080
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 600-700 of human ERCC4 (NP_005227.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GKPL RVYF LIYG GSTE EQRY LTAL RKEK EAFE KLIR EKAS MVVP EERE GRDE TNLD LVRG TASA DVST DTRK AGGQ EQNG TQQS IVVD MREF RSEL PSLI H
Gene ID:2072
Uniprot:Q92889
Cellular Location:Nucleus
Calculated MW:42kDa/104kDa
Observed MW:120kDa
Synonyms:ERCC4, ERCC11, FANCQ, RAD1, XFEPS, XPF
Background:The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).
UniProt Protein Function:Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link.
NCBI Summary:The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).[provided by RefSeq, Mar 2009]
UniProt Code:Q92889
NCBI GenInfo Identifier:4885217
NCBI Gene ID:2072
NCBI Accession:NP_005227.1
UniProt Secondary Accession:Q92889,O00140, Q8TD83, A5PKV6, A8K111,
UniProt Related Accession:Q92889
Molecular Weight:103 kDa
NCBI Full Name:DNA repair endonuclease XPF
NCBI Synonym Full Names:ERCC excision repair 4, endonuclease catalytic subunit
NCBI Official Symbol:ERCC4  
NCBI Official Synonym Symbols:XPF; RAD1; FANCQ; XFEPS; ERCC11  
NCBI Protein Information:DNA repair endonuclease XPF
UniProt Protein Name:DNA repair endonuclease XPF
UniProt Synonym Protein Names:DNA excision repair protein ERCC-4; DNA repair protein complementing XP-F cells; Xeroderma pigmentosum group F-complementing protein
Protein Family:Positive control factor
UniProt Gene Name:ERCC4  
Anti-ERCC4 Polyclonal Antibody (CAB8119)Western blot analysis of extracts of various cell lines, using ERCC4 antibody (CAB8119) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-ERCC4 Polyclonal Antibody (CAB8119)Immunoprecipitation analysis of 150ug extracts of 293T cells using 3ug ERCC4 antibody (CAB8119). Western blot was performed from the immunoprecipitate using ERCC4 antibody (CAB8119) at a dilition of 1:1000.
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