Antibody Name:	Anti-ERCC2 Antibody
Antibody SKU:	CAB5640
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ERCC2 (NP_001124339.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Rat
Positive Samples:	THP-1, 293T, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ERCC2 (NP_001124339.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MREL KRTL DAKG HGVL EMPS GTGK TVSL LALI MAYQ RAYP LEVT KLIY CSRT VPEI EKVI EELR KLLN FYEK QEGE KLPF LGLA LSSR KNLC IHPE VTPL RFGK DVDG KCHS LTAS YVRA QYQH DTSL PHCR FYEE FDAH GREV PLPA GIYN LDDL KALG RRQG WCPY FLAR YSIL HANV VVYS YHYL LDPK IADL VSKE LARK AVVV FDEA HNID NVCI DSMS VNLT RRTL DRCQ GNLE TLQK TVLR IKET DEQR LRDE YRRL VEGL REAS AARE TDAH LANP VLPD EVLQ EAVP GSIR
Gene ID:	2068
Uniprot:	P18074
Cellular Location:	Cytoplasm, Nucleus, cytoskeleton, spindle
Calculated MW:	46kDa/86kDa
Observed MW:	87kDa

Synonyms:

ERCC2, COFS2, EM9, TFIIH, TTD, TTD1, XPD

Background:

The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ERCC2: ATP-dependent 5'-3' DNA helicase, component of the core- TFIIH basal transcription factor. Involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. Might also have a role in aging process and could play a causative role in the generation of skin cancers. Belongs to the helicase family. RAD3/XPD subfamily. One of the six subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. The interaction with GTF2H2 results in the stimulation of the 5'-->3' helicase activity. Interacts with Epstein-Barr virus EBNA2.
UniProt Protein Details:	Protein type:EC 3.6.4.12; Helicase Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: nucleoplasm; holo TFIIH complex; cytoplasm; spindle; nucleus; cyclin-dependent protein kinase activating kinase holoenzyme complex Molecular Function:ATP-dependent DNA helicase activity; protein C-terminus binding; RNA polymerase subunit kinase activity; DNA-dependent ATPase activity; protein binding; DNA binding; 4 iron, 4 sulfur cluster binding; metal ion binding; protein N-terminus binding; 5'-3' DNA helicase activity; ATP binding; protein kinase activity Biological Process: transcription from RNA polymerase II promoter; extracellular matrix organization and biogenesis; erythrocyte maturation; viral reproduction; apoptosis; positive regulation of viral transcription; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of gene expression, epigenetic; bone mineralization; embryonic cleavage; post-embryonic development; chromosome segregation; mRNA capping; UV protection; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; nucleotide-excision repair, DNA damage removal; aging; positive regulation of DNA binding; transcription initiation from RNA polymerase II promoter; in utero embryonic development; multicellular organism growth; nucleotide-excision repair, DNA incision; hair cell differentiation; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; termination of RNA polymerase I transcription; DNA repair; hair follicle maturation; DNA duplex unwinding; cell proliferation; myelin formation in the central nervous system; spinal cord development; nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; gene expression; response to oxidative stress; transcription initiation from RNA polymerase I promoter Disease: Cerebrooculofacioskeletal Syndrome 2; Xeroderma Pigmentosum, Complementation Group D; Trichothiodystrophy, Photosensitive
NCBI Summary:	The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:	P18074
NCBI GenInfo Identifier:	119540
NCBI Gene ID:	2068
NCBI Accession:	P18074.1
UniProt Related Accession:	P18074
Molecular Weight:
NCBI Full Name:	General transcription and DNA repair factor IIH helicase subunit XPD
NCBI Synonym Full Names:	ERCC excision repair 2, TFIIH core complex helicase subunit
NCBI Official Symbol:	ERCC2
NCBI Official Synonym Symbols:	EM9; TTD; XPD; TTD1; COFS2; TFIIH
NCBI Protein Information:	general transcription and DNA repair factor IIH helicase subunit XPD
UniProt Protein Name:	TFIIH basal transcription factor complex helicase XPD subunit
UniProt Synonym Protein Names:	Basic transcription factor 2 80 kDa subunit; BTF2 p80; CXPD; DNA excision repair protein ERCC-2; DNA repair protein complementing XP-D cells; TFIIH basal transcription factor complex 80 kDa subunit; TFIIH 80 kDa subunit; TFIIH p80; Xeroderma pigmentosum group D-complementing protein
Protein Family:	TFIIH basal transcription factor complex helicase
UniProt Gene Name:	ERCC2
UniProt Entry Name:	ERCC2_HUMAN

	Western blot analysis of extracts of various cell lines, using ERCC2 antibody (CAB5640) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of HeLa cells using ERCC2 antibody (CAB5640). Blue: DAPI for nuclear staining.