Epigenetics & Nuclear Signaling Antibodies 3
Anti-ERCC1 Antibody (CAB5291)
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- SKU:
- CAB5291
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-ERCC1 Antibody |
| Antibody SKU: | CAB5291 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human ERCC1 (NP_973730.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Raji, MCF-7, PC-3, HepG2, HeLa, Mouse liver, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human ERCC1 (NP_973730.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDPG KDKE GVPQ PSGP PARK KFVI PLDE DEVP PGVA KPLF RSTQ SLPT VDTS AQAA PQTY AEYA ISQP LEGA GATC PTGS EPLA GETP NQAL KPGA KSNS IIVS PRQR GNPV LKFV RNVP WEFG DVIP DYVL GQST CALF LSLR YHNL HPDY IHGR LQSL GKNF ALRV LLVQ VDVK DPQQ ALKE LAKM CILA DCTL ILAW SPEE AGRY LETY KAYE QKPA DLLM EKLE QDFV SRVT ECLT TVKS VNKT DSQT LLTT FGSL EQLI AASR EDLA LCPG LGPQ KVRA LGKN PRSW GKER APNK HNLR PQSF KVKK EPKT RHSG FRL |
| Gene ID: | 2067 |
| Uniprot: | P07992 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 25kDa/29kDa/32kDa/35kDa |
| Observed MW: | 38kDa |
| Synonyms: | ERCC1, COFS4, RAD10, UV20 |
| Background: | The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. |
| UniProt Protein Function: | Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4. |
| NCBI Summary: | The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009] |
| UniProt Code: | P07992 |
| NCBI GenInfo Identifier: | 119538 |
| NCBI Gene ID: | 2067 |
| NCBI Accession: | P07992.1 |
| UniProt Secondary Accession: | P07992,Q7Z7F5, Q96S40, B2RC01, B3KRR0, |
| UniProt Related Accession: | P07992 |
| Molecular Weight: | 25,211 Da |
| NCBI Full Name: | DNA excision repair protein ERCC-1 |
| NCBI Synonym Full Names: | ERCC excision repair 1, endonuclease non-catalytic subunit |
| NCBI Official Symbol: | ERCC1 |
| NCBI Official Synonym Symbols: | UV20; COFS4; RAD10 |
| NCBI Protein Information: | DNA excision repair protein ERCC-1 |
| UniProt Protein Name: | DNA excision repair protein ERCC-1 |
| Protein Family: | DNA excision repair protein |
| UniProt Gene Name: | ERCC1 |
| UniProt Entry Name: | ERCC1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ERCC1 antibody (CAB5291) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using ERCC1 antibody (CAB5291). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using ERCC1 antibody (CAB5291). |
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