Name: Anti-Erbin Antibody (CAB8585)
Brand: Cell Biology Antibodies 12
SKU: CAB8585
Availability: OutOfStock

Antibody Name:	Anti-Erbin Antibody
Antibody SKU:	CAB8585
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1152-1371 of human ERBB2IP (NP_061165.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	293T, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1152-1371 of human ERBB2IP (NP_061165.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IPER TMSV SDFN YSRT SPSK RPNA RVGS EHSL LDPP GKSK VPRD WREQ VLRH IEAK KLEK MPLS NGQM GQPL RPQA NYSQ IHHP PQAS VARH PSRE QLID YLML KVAH QPPY TQPH CSPR QGHE LAKQ EIRV RVEK DPEL GFSI SGGV GGRG NPFR PDDD GIFV TRVQ PEGP ASKL LQPG DKII QANG YSFI NIEH GQAV SLLK TFQN TVEL IIVR EVSS
Gene ID:	55914
Uniprot:	Q96RT1
Cellular Location:	Basolateral cell membrane, Cell junction, Nucleus membrane, hemidesmosome
Calculated MW:	146-159kDa
Observed MW:	182kDa

Synonyms:

ERBIN, ERBB2IP, HEL-S-78, LAP2, erbin

Background:

This gene is a member of the leucine-rich repeat and PDZ domain (LAP) family. The encoded protein contains 17 leucine-rich repeats and one PDZ domain. It binds to the unphosphorylated form of the ERBB2 protein and regulates ERBB2 function and localization. It has also been shown to affect the Ras signaling pathway by disrupting Ras-Raf interaction. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	Erbin: Acts as an adapter for the receptor ERBB2, in epithelia. By binding the unphosphorylated 'Tyr-1248' of receptor ERBB2, it may contribute to stabilize this unphosphorylated state. Belongs to the LAP (LRR and PDZ) protein family. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell adhesion Chromosomal Location of Human Ortholog: 5q12.3 Cellular Component: nucleoplasm; nuclear membrane; hemidesmosome; cytoplasm; basal plasma membrane; plasma membrane; basement membrane; cell junction; nucleus Molecular Function:integrin binding; ErbB-2 class receptor binding; protein binding; structural constituent of cytoskeleton Biological Process: integrin-mediated signaling pathway; establishment and/or maintenance of epithelial cell polarity; epidermal growth factor receptor signaling pathway; basal protein localization; intermediate filament cytoskeleton organization and biogenesis; cell adhesion; cell growth; signal transduction; cell cycle; protein targeting
NCBI Summary:	This gene is a member of the leucine-rich repeat and PDZ domain (LAP) family. The encoded protein contains 17 leucine-rich repeats and one PDZ domain. It binds to the unphosphorylated form of the ERBB2 protein and regulates ERBB2 function and localization. It has also been shown to affect the Ras signaling pathway by disrupting Ras-Raf interaction. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
UniProt Code:	Q96RT1
NCBI GenInfo Identifier:	8923909
NCBI Gene ID:	55914
NCBI Accession:	NP_061165.1
UniProt Related Accession:	Q96RT1
Molecular Weight:
NCBI Full Name:	erbin isoform 2
NCBI Synonym Full Names:	erbb2 interacting protein
NCBI Official Symbol:	ERBIN
NCBI Official Synonym Symbols:	LAP2; ERBB2IP; HEL-S-78
NCBI Protein Information:	erbin
UniProt Protein Name:	Protein LAP2
UniProt Synonym Protein Names:	Densin-180-like protein; Erbb2-interacting protein; Erbin
Protein Family:	Leucine aminopeptidase
UniProt Gene Name:	ERBB2IP
UniProt Entry Name:	LAP2_HUMAN

Anti-Erbin Polyclonal Antibody (CAB8585)

Western blot analysis of extracts of various cell lines, using ERBB2IP antibody (CAB8585) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Phospho-ERBB2IP (Y1104) Antibody (PACO00207)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)