Name: Anti-EPRS Antibody (CAB15245)
Brand: Metabolism Antibodies 1
SKU: CAB15245
Availability: OutOfStock

Antibody Name:	Anti-EPRS Antibody
Antibody SKU:	CAB15245
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human EPRS (NP_004437.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100
Reactivity:	Human
Positive Samples:	293T, HeLa

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human EPRS (NP_004437.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EAKV LFDK VASQ GEVV RKLK TEKA PKDQ VDIA VQEL LQLK AQYK SLIG VEYK PVSA TGAE DKDK KKKE KENK SEKQ NKPQ KQND GQRK DPSK NQGG GLSS S
Gene ID:	2058
Uniprot:	P07814
Cellular Location:	Cytoplasm
Calculated MW:	170kDa
Observed MW:	190kDa

Synonyms:

EPRS, EARS, GLUPRORS, PARS, PIG32, QARS, QPRS

Background:

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined.

UniProt Protein Function:	EPRS: Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43. Interacts with DUS2L.
UniProt Protein Details:	Protein type:Translation; EC 6.1.1.17; Ligase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 6.1.1.15 Chromosomal Location of Human Ortholog: 1q41 Cellular Component: cytoplasm; cytosol; membrane; ribonucleoprotein complex Molecular Function:glutamate-tRNA ligase activity; GTPase binding; proline-tRNA ligase activity; protein binding Biological Process: negative regulation of translation; prolyl-tRNA aminoacylation; protein complex assembly; tRNA aminoacylation for protein translation
NCBI Summary:	Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	P07814
NCBI GenInfo Identifier:	288558855
NCBI Gene ID:	2058
NCBI Accession:	P07814.5
UniProt Secondary Accession:	P07814,Q05BP6, Q05DF8, Q5DSM1, Q5H9S5, Q6PD57, Q86X73 A0AVA9, B9EGH3,
UniProt Related Accession:	P07814
Molecular Weight:	170,591 Da
NCBI Full Name:	Bifunctional glutamate/proline--tRNA ligase
NCBI Synonym Full Names:	glutamyl-prolyl-tRNA synthetase
NCBI Official Symbol:	EPRS
NCBI Official Synonym Symbols:	EARS; PARS; QARS; QPRS; PIG32; GLUPRORS
NCBI Protein Information:	bifunctional glutamate/proline--tRNA ligase
UniProt Protein Name:	Bifunctional glutamate/proline--tRNA ligase
UniProt Synonym Protein Names:	Bifunctional aminoacyl-tRNA synthetase; Cell proliferation-inducing gene 32 protein; Glutamatyl-prolyl-tRNA synthetaseIncluding the following 2 domains:Glutamate--tRNA ligase (EC:6.1.1.17)Alternative name(s):Glutamyl-tRNA synthetase; GluRS
UniProt Gene Name:	EPRS
UniProt Entry Name:	SYEP_HUMAN

Western blot analysis of extracts of various cell lines, using EPRS antibody (CAB15245) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

ELISA Kits

Human EPRS (glutamyl-prolyl-tRNA synthetase ) ELISA Kit (HUFI04413)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)