Antibody Name:	Anti-EPPIN Antibody
Antibody SKU:	CAB2149
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-133 of human EPPIN (NP_065131.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-133 of human EPPIN (NP_065131.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PGLT DWLF PRRC PKIR EECE FQER DVCT KDRQ CQDN KKCC VFSC GKKC LDLK QDVC EMPK ETGP CLAY FLHW WYDK KDNT CSMF VYGG CQGN NNNF QSKA NCLN TCKN KRFP
Gene ID:	57119
Uniprot:	O95925
Cellular Location:	Cell surface, Secreted
Calculated MW:	13kDa/15kDa/20kDa
Observed MW:	25kDa

Synonyms:

EPPIN, CT71, CT72, SPINLW1, WAP7, WFDC7, dJ461P17.2, eppin

Background:

This gene encodes an epididymal protease inhibitor, which contains both kunitz-type and WAP-type four-disulfide core (WFDC) protease inhibitor consensus sequences. Most WFDC genes are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene is a member of the WFDC gene family and belongs to the telomeric cluster. The protein can inhibit human sperm motility and exhibits antimicrobial activity against E. coli, and polymorphisms in this gene are associated with male infertility. Read-through transcription also exists between this gene and the downstream WFDC6 (WAP four-disulfide core domain 6) gene. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	Spinlw1: Serine protease inhibitor that plays an essential role in male reproduction and fertility. Modulates the hydrolysis of SEMG1 by KLK3/PSA (a serine protease), provides antimicrobial protection for spermatozoa in the ejaculate coagulum, and binds SEMG1 thereby inhibiting sperm motility. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Inhibitor; Secreted; Secreted, signal peptide; Cancer Testis Antigen (CTA) Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: cell surface; extracellular region Molecular Function:serine-type endopeptidase inhibitor activity; protein binding Biological Process: defense response to bacterium; negative regulation of peptidase activity
NCBI Summary:	This gene encodes an epididymal protease inhibitor, which contains both kunitz-type and WAP-type four-disulfide core (WFDC) protease inhibitor consensus sequences. Most WFDC genes are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene is a member of the WFDC gene family and belongs to the telomeric cluster. The protein can inhibit human sperm motility and exhibits antimicrobial activity against E. coli, and polymorphisms in this gene are associated with male infertility. Read-through transcription also exists between this gene and the downstream WFDC6 (WAP four-disulfide core domain 6) gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
UniProt Code:	O95925
NCBI GenInfo Identifier:	13959368
NCBI Gene ID:	57119
NCBI Accession:	O95925.1
UniProt Secondary Accession:	O95925,Q86TP9, Q96SD7, Q9HD30, A6PVD6,
UniProt Related Accession:	O95925
Molecular Weight:	133
NCBI Full Name:	Eppin
NCBI Synonym Full Names:	epididymal peptidase inhibitor
NCBI Official Symbol:	EPPIN
NCBI Official Synonym Symbols:	CT71; CT72; WAP7; WFDC7; SPINLW1; dJ461P17.2
NCBI Protein Information:	eppin; protease inhibitor WAP7; cancer/testis antigen 71; cancer/testis antigen 72; epididymal protease inhibitor; WAP four-disulfide core domain protein 7; serine peptidase inhibitor-like, with Kunitz and WAP domains 1 (eppin)
UniProt Protein Name:	Eppin
UniProt Synonym Protein Names:	Cancer/testis antigen 71; CT71; Epididymal protease inhibitor; Protease inhibitor WAP7; Serine protease inhibitor-like with Kunitz and WAP domains 1; WAP four-disulfide core domain protein 7
Protein Family:	Eppin
UniProt Gene Name:	EPPIN
UniProt Entry Name:	EPPI_HUMAN

	Western blot analysis of extracts of mouse testis, using EPPIN antibody (CAB2149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
	Immunofluorescence analysis of HepG2 cells using EPPIN antibody (CAB2149). Blue: DAPI for nuclear staining.