Cell Biology Antibodies 8

Anti-EPOR Antibody (CAB2917)

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SKU:
CAB2917
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-EPOR Antibody
Antibody SKU:CAB2917
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 274-508 of human EPOR (NP_000112.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:K-562, Mouse kidney, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 274-508 of human EPOR (NP_000112.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HRRA LKQK IWPG IPSP ESEF EGLF TTHK GNFQ LWLY QNDG CLWW SPCT PFTE DPPA SLEV LSER CWGT MQAV EPGT DDEG PLLE PVGS EHAQ DTYL VLDK WLLP RNPP SEDL PGPG GSVD IVAM DEGS EASS CSSA LASK PSPE GASA ASFE YTIL DPSS QLLR PWTL CPEL PPTP PHLK YLYL VVSD SGIS TDYS SGDS QGAQ GGLS DGPY SNPY ENSL IPAA EPLP PSYV ACS
Gene ID:2057
Uniprot:P19235
Cellular Location:Cell membrane, Secreted, Single-pass type I membrane protein
Calculated MW:26kDa/35kDa/55kDa
Observed MW:55kDa
Synonyms:EPOR, EPO-R
Background:This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.
UniProt Protein Details:

Protein type:Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: integral to plasma membrane

Molecular Function:erythropoietin receptor activity; identical protein binding; protein binding

Disease: Erythrocytosis, Familial, 1

NCBI Summary:This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
UniProt Code:P19235
NCBI GenInfo Identifier:119524
NCBI Gene ID:2057
NCBI Accession:P19235.1
UniProt Secondary Accession:P19235,Q15443, Q2M205, B2RCG4,
UniProt Related Accession:P19235
Molecular Weight:35,809 Da
NCBI Full Name:Erythropoietin receptor
NCBI Synonym Full Names:erythropoietin receptor
NCBI Official Symbol:EPOR  
NCBI Official Synonym Symbols:EPO-R  
NCBI Protein Information:erythropoietin receptor
UniProt Protein Name:Erythropoietin receptor
Protein Family:Erythropoietin receptor
UniProt Gene Name:EPOR  
UniProt Entry Name:EPOR_HUMAN
Anti-EPOR Antibody (CAB2917)Western blot analysis of extracts of various cell lines, using EPOR antibody (CAB2917) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
Anti-EPOR Antibody (CAB2917)Immunohistochemistry of paraffin-embedded human prostate using EPOR antibody (CAB2917) at dilution of 1:100 (40x lens).
Anti-EPOR Antibody (CAB2917)Immunohistochemistry of paraffin-embedded human trachea using EPOR antibody (CAB2917) at dilution of 1:100 (40x lens).
Anti-EPOR Antibody (CAB2917)Immunofluorescence analysis of Mouse fetal using EPOR Rabbit pAb (CAB2917) at dilution of 1:100. Blue: DAPI for nuclear staining.
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