Neuroscience

Anti-EPHA5 Antibody (CAB14238)

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SKU:
CAB14238
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Neuroscience

Description

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Antibody Name:Anti-EPHA5 Antibody
Antibody SKU:CAB14238
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human EPHA5 (NP_004430.4).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, U-251MG, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human EPHA5 (NP_004430.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MRGS GPRG AGRR RPPS GGGD TPIT PASL AGCY SAPR RAPL WTCL LLCA ALRT LLAS PSNE VNLL DSRT VMGD LGWI AFPK NGWE EIGE VDEN YAPI HTYQ VCKV MEQN QNNW LLTS WISN EGAS RIFI EL
Gene ID:2044
Uniprot:P54756
Cellular Location:Cell membrane, Cell projection, Single-pass type I membrane protein, axon, dendrite
Calculated MW:107kDa/112kDa/114kDa
Observed MW:115kDa
Synonyms:EPHA5, CEK7, EHK-1, EHK1, EK7, HEK7, TYRO4
Background:This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described.
UniProt Protein Function:EphA5: Receptor tyrosine kinase which binds promiscuously GPI- anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino- hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. Beside its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Protein kinase, tyrosine (receptor); Kinase, protein; Membrane protein, integral; Protein kinase, TK; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 4q13.1

Cellular Component: cell soma; dendrite; external side of plasma membrane; perinuclear region of cytoplasm; plasma membrane; rough endoplasmic reticulum

Molecular Function:ephrin receptor activity; GPI-linked ephrin receptor activity

Biological Process: activation of CREB transcription factor; axon guidance; cAMP-mediated signaling; ephrin receptor signaling pathway; hippocampus development; neuron development; regulation of actin cytoskeleton organization and biogenesis; regulation of GTPase activity

NCBI Summary:This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]
UniProt Code:P54756
NCBI GenInfo Identifier:259016353
NCBI Gene ID:2044
NCBI Accession:P54756.3
UniProt Secondary Accession:P54756,Q7Z3F2,
UniProt Related Accession:P54756
Molecular Weight:107,699 Da
NCBI Full Name:Ephrin type-A receptor 5
NCBI Synonym Full Names:EPH receptor A5
NCBI Official Symbol:EPHA5  
NCBI Official Synonym Symbols:EK7; CEK7; EHK1; HEK7; EHK-1; TYRO4  
NCBI Protein Information:ephrin type-A receptor 5
UniProt Protein Name:Ephrin type-A receptor 5
UniProt Synonym Protein Names:Brain-specific kinase; EPH homology kinase 1; EHK-1; EPH-like kinase 7; EK7; hEK7
Protein Family:Ephrin type-A receptor
UniProt Gene Name:EPHA5  
UniProt Entry Name:EPHA5_HUMAN
Anti-EPHA5 Antibody (CAB14238)Western blot analysis of extracts of various cell lines, using EPHA5 antibody (CAB14238) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-EPHA5 Antibody (CAB14238)Immunofluorescence analysis of L929 cells using EPHA5 Rabbit pAb (CAB14238) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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