Cell Biology Antibodies 12

Anti-EPHA3 Antibody (CAB8414)

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SKU:
CAB8414
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-EPHA3 Antibody
Antibody SKU:CAB8414
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-545 of human EPHA3 (NP_005224.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SW620, Mouse lung, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-545 of human EPHA3 (NP_005224.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LAHT NYTF EIDA VNGV SELS SPPR QFAA VSIT TNQA APSP VLTI KKDR TSRN SISL SWQE PEHP NGII LDYE VKYY EKQE QETS YTIL RARG TNVT ISSL KPDT IYVF QIRA RTAA GYGT NSRK FEFE TSPD SFSI SGES SQVV MI
Gene ID:2042
Uniprot:P29320
Cellular Location:Cell membrane, Secreted, Single-pass type I membrane protein
Calculated MW:60kDa/110kDa
Observed MW:110kDa
Synonyms:EK4, ETK, HEK, ETK1, HEK4, TYRO4, EPHA3
Background:This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene.
UniProt Protein Function:EphA3: a receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-A family: binds to ephrin-A2, -A3, -A4 and -A5The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Two point mutations seen in a survey of colorectal tumors. Soluble receptors reduce tumor growth and angiogenesis in mouse models. Two alternatively spliced isoforms of EphA3 have been described.
UniProt Protein Details:

Protein type:EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; Kinase, protein; TK group; Eph family

Chromosomal Location of Human Ortholog: 3p11.2

Cellular Component: early endosome; extracellular region; integral to plasma membrane; plasma membrane

Molecular Function:ATP binding; GPI-linked ephrin receptor activity; protein binding

Biological Process: cell adhesion; cell migration; ephrin receptor signaling pathway; peptidyl-tyrosine phosphorylation; regulation of actin cytoskeleton organization and biogenesis; regulation of focal adhesion formation; regulation of GTPase activity

NCBI Summary:This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P29320
NCBI GenInfo Identifier:116241351
NCBI Gene ID:2042
NCBI Accession:P29320.2
UniProt Secondary Accession:P29320,Q9H2V3, Q9H2V4,
UniProt Related Accession:P29320
Molecular Weight:60,946 Da
NCBI Full Name:Ephrin type-A receptor 3
NCBI Synonym Full Names:EPH receptor A3
NCBI Official Symbol:EPHA3  
NCBI Official Synonym Symbols:EK4; ETK; HEK; ETK1; HEK4; TYRO4  
NCBI Protein Information:ephrin type-A receptor 3
UniProt Protein Name:Ephrin type-A receptor 3
UniProt Synonym Protein Names:EPH-like kinase 4; EK4; hEK4; HEK; Human embryo kinase; Tyrosine-protein kinase TYRO4; Tyrosine-protein kinase receptor ETK1; Eph-like tyrosine kinase 1
Protein Family:Ephrin type-A receptor
UniProt Gene Name:EPHA3  
UniProt Entry Name:EPHA3_HUMAN
Anti-EPHA3 Polyclonal Antibody (CAB8414)Western blot analysis of extracts of various cell lines, using EPHA3 antibody (CAB8414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-EPHA3 Polyclonal Antibody (CAB8414)Immunohistochemistry of paraffin-embedded rat brain using EPHA3 antibody (CAB8414) at dilution of 1:100 (40x lens).
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