Cell Biology Antibodies 2
Anti-EpCAM Antibody (CAB1177)
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- SKU:
- CAB1177
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-EpCAM Antibody |
| Antibody SKU: | CAB1177 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human EpCAM (NP_002345.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | NIH/3T3, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human EpCAM (NP_002345.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VNNN RQCQ CTSV GAQN TVIC SKLA AKCL VMKA EMNG SKLG RRAK PEGA LQNN DGLY DPDC DESG LFKA KQCN GTSM CWCV NTAG VRRT DKDT EITC SERV RTYW IIIE LKHK AREK PYDS KSLR TALQ KEIT TRYQ LDPK FITS ILYE NNVI TIDL VQNS SQKT QNDV DIAD VAYY FEKD VKGE SLFH SKKM DLTV NGEQ LDLD PGQT LIYY VDEK APEF S |
| Gene ID: | 4072 |
| Uniprot: | P16422 |
| Cellular Location: | Cell junction, Lateral cell membrane, Single-pass type I membrane protein, tight junction |
| Calculated MW: | 34kDa |
| Observed MW: | 40kDa |
| Synonyms: | EPCAM, DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1 |
| Background: | This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. |
| UniProt Protein Function: | EPCAM: a single-pass type I membrane protein of the EPCAM family. May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinomas including pancreatic carcinoma. May be a marker of cancer stem cells. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Motility/polarity/chemotaxis Cellular Component: tight junction; cell surface; membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane; integral to membrane; cell junction; lateral plasma membrane Molecular Function:protein complex binding Biological Process: positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; stem cell differentiation; negative regulation of apoptosis |
| UniProt Code: | P16422 |
| NCBI GenInfo Identifier: | 112293275 |
| NCBI Gene ID: | 17075 |
| NCBI Accession: | NP_032558.2 |
| UniProt Secondary Accession: | P16422,Q61512, |
| UniProt Related Accession: | Q99JW5 |
| Molecular Weight: | |
| NCBI Full Name: | epithelial cell adhesion molecule |
| NCBI Synonym Full Names: | epithelial cell adhesion molecule |
| NCBI Official Symbol: | Epcam |
| NCBI Official Synonym Symbols: | EGP; Ly74; gp40; CD326; EGP-2; TROP1; Egp314; Ep-CAM; EpCAM1; Tacsd1; GA733-2; Tacstd1 |
| NCBI Protein Information: | epithelial cell adhesion molecule |
| UniProt Protein Name: | Epithelial cell adhesion molecule |
| UniProt Synonym Protein Names: | Epithelial glycoprotein 314; EGP314; mEGP314; Protein 289A; Tumor-associated calcium signal transducer 1; CD_antigen: CD326 |
| Protein Family: | Epithelial cell adhesion molecule |
| UniProt Gene Name: | Epcam |
| UniProt Entry Name: | EPCAM_MOUSE |
 | Western blot analysis of extracts of various cell lines, using EpCAM antibody (CAB1177) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human lung cancer using EpCAM antibody (CAB1177) at dilution of 1:100 (40x lens). |
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