Name: Anti-ENTPD6 Antibody (CAB15655)
Brand: Cell Biology Antibodies 6
SKU: CAB15655
Availability: OutOfStock

Antibody Name:	Anti-ENTPD6 Antibody
Antibody SKU:	CAB15655
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 61-300 of human ENTPD6 (NP_001304870.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Mouse spleen, Rat brain, Rat spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 61-300 of human ENTPD6 (NP_001304870.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	WHRA TATQ AFFS ITRA APGA RWGQ QAHS PLGT AADG HEVF YGIM FDAG STGT RVHV FQFT RPPR ETPT LTHE TFKA LKPG LSAY ADDV EKSA QGIR ELLD VAKQ DIPF DFWK ATPL VLKA TAGL RLLP GEKA QKLL QKVK EVFK ASPF LVGD DCVS IMNG TDEG VSAW ITIN FLTG SLKT PGGS SVGM LDLG GGST QIAF LPRV EGTL QASP PGYL TALR MFNR TYKL YSYS YLGL GLMS
Gene ID:	955
Uniprot:	O75354
Cellular Location:	Golgi apparatus membrane, Secreted, Single-pass type II membrane protein
Calculated MW:	51kDa/53kDa
Observed MW:	45kDa

Synonyms:	ENTPD6, CD39L2, IL-6SAG, IL6ST2, NTPDase-6, dJ738P15.3
Background:	ENTPD6 is similar to E-type nucleotidases (NTPases). NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD6 contains 4 apyrase-conserved regions which are characteristic of NTPases. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	ENTPD6: Might support glycosylation reactions in the Golgi apparatus and, when released from cells, might catalyze the hydrolysis of extracellular nucleotides. Hydrolyzes preferentially nucleoside 5'-diphosphates, nucleoside 5'-triphosphates are hydrolyzed only to a minor extent, there is no hydrolysis of nucleoside 5'-monophosphates. The order of activity with different substrates is GDP > IDP >> UDP = CDP >> ADP. Belongs to the GDA1/CD39 NTPase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Membrane protein, integral; Hydrolase; EC 3.6.1.6 Chromosomal Location of Human Ortholog: 20p11.21 Cellular Component: Golgi membrane; extracellular space; cell surface; integral to membrane; plasma membrane Molecular Function:nucleoside-triphosphatase activity; guanosine-5'-triphosphate,3'-diphosphate diphosphatase activity; uridine-diphosphatase activity Biological Process: response to magnesium ion; metabolic process; response to calcium ion
NCBI Summary:	ENTPD6 is similar to E-type nucleotidases (NTPases). NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD6 contains 4 apyrase-conserved regions which is characteristic of NTPases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	O75354
NCBI GenInfo Identifier:	317373443
NCBI Gene ID:	955
NCBI Accession:	O75354.3
UniProt Secondary Accession:	O75354,Q5QPJ2, Q5QPJ5, Q7Z5B5, Q8N3H3, Q8TAS7, Q9UJD1 A6NCX6, D3DW49,
UniProt Related Accession:	O75354
Molecular Weight:	53,118 Da
NCBI Full Name:	Ectonucleoside triphosphate diphosphohydrolase 6
NCBI Synonym Full Names:	ectonucleoside triphosphate diphosphohydrolase 6 (putative)
NCBI Official Symbol:	ENTPD6
NCBI Official Synonym Symbols:	CD39L2; IL6ST2; IL-6SAG; NTPDase-6; dJ738P15.3
NCBI Protein Information:	ectonucleoside triphosphate diphosphohydrolase 6; CD39 antigen-like 2; CD39-like 2; HCV-E2 binding protein 1; NTPDase 6; ectonucleoside triphosphate diphosphohydrolase 6 (putative function); interleukin 6 signal transducer-2
UniProt Protein Name:	Ectonucleoside triphosphate diphosphohydrolase 6
UniProt Synonym Protein Names:	CD39 antigen-like 2
Protein Family:	Ectonucleoside triphosphate diphosphohydrolase
UniProt Gene Name:	ENTPD6
UniProt Entry Name:	ENTP6_HUMAN

	Western blot analysis of extracts of various cell lines, using ENTPD6 antibody (CAB15655) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using ENTPD6 antibody (CAB15655) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using ENTPD6 antibody (CAB15655) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spinal cord using ENTPD6 antibody (CAB15655) at dilution of 1:100 (40x lens).