Name: Anti-ENTPD4 Antibody (CAB16469)
Brand: Cell Biology Antibodies 7
SKU: CAB16469
Availability: OutOfStock

Antibody Name:	Anti-ENTPD4 Antibody
Antibody SKU:	CAB16469
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 350-470 of human ENTPD4 (NP_001122402.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, Mouse heart, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 350-470 of human ENTPD4 (NP_001122402.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LTPD MPYL DPCL PLDI KDEI QQNG QTIY LRGT GDFD LCRE TIQP FMNK TNET QTSL NGVY QPPI HFQN SEFY GFSE FYYC TEDV LRMG GDYN AAKF TKAA KDYC ATKW SILR ERFD RGLY A
Gene ID:	9583
Uniprot:	Q9Y227
Cellular Location:	Cytoplasmic vesicle, Golgi apparatus membrane, Multi-pass membrane protein, autophagosome membrane
Calculated MW:	69kDa/70kDa
Observed MW:	80kDa

Synonyms:

ENTPD4, LALP70, LAP70, LYSAL1, NTPDase-4, UDPase

Background:

This gene encodes a member of the apyrase protein family. Apyrases are enzymes that catalyze the hydrolysis of nucleotide diphosphates and triphosphates in a calcium or magnesium-dependent manner. The encoded protein is an endo-apyrase and may play a role in salvaging nucleotides from lysosomes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and these isoforms may differ in divalent cation dependence and substrate specificity.

UniProt Protein Function:	UDPase: an ectonucleoside triphosphate diphosphohydrolase. Hydrolyzes preferentially nucleoside 5'-diphosphates; nucleoside 5'-triphosphates are hydrolyzed only to a minor extent. The order of activity with different substrates is UDP >> GDP = CDP = TDP; AMP, ADP, ATP and UMP are not substrates. Two splice variant isoforms have been described. Preferred substrates for isoform 2 are CTP, UDP, CDP, GTP and GDP, while isoform 1 utilizes UTP and TTP
UniProt Protein Details:	Protein type:EC 3.6.1.6; Nucleotide Metabolism - pyrimidine; Hydrolase; Membrane protein, multi-pass; Nucleotide Metabolism - purine; Membrane protein, integral Chromosomal Location of Human Ortholog: 8p21.3 Cellular Component: integral to Golgi membrane; intracellular membrane-bound organelle Molecular Function:uridine-diphosphatase activity Biological Process: UDP catabolic process
NCBI Summary:	This gene encodes a member of the apyrase protein family. Apyrases are enzymes that catalyze the hydrolysis of nucleotide diphosphates and triphosphates in a calcium or magnesium-dependent manner. The encoded protein is an endo-apyrase and may play a role in salvaging nucleotides from lysosomes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and these isoforms may differ in divalent cation dependence and substrate specificity. [provided by RefSeq, Sep 2011]
UniProt Code:	Q9Y227
NCBI GenInfo Identifier:	18203627
NCBI Gene ID:	9583
NCBI Accession:	Q9Y227.1
UniProt Secondary Accession:	Q9Y227,O15092, D3DSS3,
UniProt Related Accession:	Q9Y227
Molecular Weight:	69,421 Da
NCBI Full Name:	Ectonucleoside triphosphate diphosphohydrolase 4
NCBI Synonym Full Names:	ectonucleoside triphosphate diphosphohydrolase 4
NCBI Official Symbol:	ENTPD4
NCBI Official Synonym Symbols:	LAP70; LALP70; LYSAL1; UDPase; NTPDase-4
NCBI Protein Information:	ectonucleoside triphosphate diphosphohydrolase 4
UniProt Protein Name:	Ectonucleoside triphosphate diphosphohydrolase 4
UniProt Synonym Protein Names:	Lysosomal apyrase-like protein of 70 kDa; Uridine-diphosphatase; UDPase
Protein Family:	Ectonucleoside triphosphate diphosphohydrolase
UniProt Gene Name:	ENTPD4
UniProt Entry Name:	ENTP4_HUMAN

Western blot analysis of extracts of various cell lines, using ENTPD4 antibody (CAB16469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

ENTPD4 Antibody (PACO09103)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)