Metabolism Antibodies 2

Anti-ENO2 Antibody (CAB3118)

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SKU:
CAB3118
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-ENO2 Antibody
Antibody SKU:CAB3118
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide of human ENO2
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, OVCAR3, 22Rv1, SW620, A-549, Jurkat, Mouse brain, Mouse spinal cord, Mouse eye
Immunogen:A synthetic peptide of human ENO2
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:2026
Uniprot:P09104
Cellular Location:Cell membrane, Cytoplasm
Calculated MW:42kDa/47kDa
Observed MW:50kDa
Synonyms:ENO2, HEL-S-279, NSE, enolase 2
Background:This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
UniProt Protein Function:ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytosol; extracellular space; myelin sheath; perikaryon; phosphopyruvate hydratase complex; photoreceptor inner segment; plasma membrane

Molecular Function:magnesium ion binding; phosphopyruvate hydratase activity; protein binding

Biological Process: carbohydrate metabolic process; gluconeogenesis; glucose metabolic process

NCBI Summary:This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
UniProt Code:P09104
NCBI GenInfo Identifier:20981682
NCBI Gene ID:2026
NCBI Accession:P09104.3
UniProt Secondary Accession:P09104,Q96J33, B7Z2X9,
UniProt Related Accession:P09104
Molecular Weight:42,707 Da
NCBI Full Name:Gamma-enolase
NCBI Synonym Full Names:enolase 2 (gamma, neuronal)
NCBI Official Symbol:ENO2  
NCBI Official Synonym Symbols:NSE; HEL-S-279  
NCBI Protein Information:gamma-enolase
UniProt Protein Name:Gamma-enolase
UniProt Synonym Protein Names:2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE
Protein Family:Gamma-enolase
UniProt Gene Name:ENO2  
UniProt Entry Name:ENOG_HUMAN
Anti-ENO2 Antibody (CAB3118)Western blot analysis of extracts of various cell lines, using ENO2 antibody (CAB3118) at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 90S.
Anti-ENO2 Antibody (CAB3118)Immunohistochemistry of paraffin-embedded rat brain using ENO2 antibody (CAB3118) at dilution of 1:100 (40x lens).
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