Metabolism Antibodies 1
Anti-ENO2 Antibody (CAB12341)
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- SKU:
- CAB12341
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-ENO2 Antibody |
| Antibody SKU: | CAB12341 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 195-434 of human ENO2 (NP_001966.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, LO2, SH-SY5Y, U-251MG, Mouse skeletal muscle, Mouse liver, Mouse brain, Rat brain, Rat skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 195-434 of human ENO2 (NP_001966.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VIKD KYGK DATN VGDE GGFA PNIL ENSE ALEL VKEA IDKA GYTE KIVI GMDV AASE FYRD GKYD LDFK SPTD PSRY ITGD QLGA LYQD FVRD YPVV SIED PFDQ DDWA AWSK FTAN VGIQ IVGD DLTV TNPK RIER AVEE KACN CLLL KVNQ IGSV TEAI QACK LAQE NGWG VMVS HRSG ETED TFIA DLVV GLCT GQIK TGAP CRSE RLAK YNQL MRIE EELG DEAR FAGH NFRN PSVL |
| Gene ID: | 2026 |
| Uniprot: | P09104 |
| Cellular Location: | Cell membrane, Cytoplasm |
| Calculated MW: | 42kDa/47kDa |
| Observed MW: | 47kDa |
| Synonyms: | ENO2, HEL-S-279, NSE, enolase 2 |
| Background: | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. |
| UniProt Protein Function: | ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase Chromosomal Location of Human Ortholog: 12p13 Cellular Component: cytosol; extracellular space; myelin sheath; perikaryon; phosphopyruvate hydratase complex; photoreceptor inner segment; plasma membrane Molecular Function:magnesium ion binding; phosphopyruvate hydratase activity; protein binding Biological Process: carbohydrate metabolic process; gluconeogenesis; glucose metabolic process |
| NCBI Summary: | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P09104 |
| NCBI GenInfo Identifier: | 20981682 |
| NCBI Gene ID: | 2026 |
| NCBI Accession: | P09104.3 |
| UniProt Secondary Accession: | P09104,Q96J33, B7Z2X9, |
| UniProt Related Accession: | P09104 |
| Molecular Weight: | 42,707 Da |
| NCBI Full Name: | Gamma-enolase |
| NCBI Synonym Full Names: | enolase 2 (gamma, neuronal) |
| NCBI Official Symbol: | ENO2 |
| NCBI Official Synonym Symbols: | NSE; HEL-S-279 |
| NCBI Protein Information: | gamma-enolase |
| UniProt Protein Name: | Gamma-enolase |
| UniProt Synonym Protein Names: | 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE |
| Protein Family: | Gamma-enolase |
| UniProt Gene Name: | ENO2 |
| UniProt Entry Name: | ENOG_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ENO2 antibody (CAB12341) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of L929 cells using ENO2 antibody (CAB12341) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of PC-12 cells using ENO2 antibody (CAB12341) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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