KO Validated Antibodies 1

Anti-ENO1 Antibody (CAB1033)[KO Validated]

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SKU:
CAB1033
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-ENO1 Antibody [KO Validated]
Antibody SKU:CAB1033
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-434 of human ENO1 (NP_001419.1).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:PC-3, Ramos, NCI-H460, HeLa, THP-1, Mouse kidney, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-434 of human ENO1 (NP_001419.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSIL KIHA REIF DSRG NPTV EVDL FTSK GLFR AAVP SGAS TGIY EALE LRDN DKTR YMGK GVSK AVEH INKT IAPA LVSK KLNV TEQE KIDK LMIE MDGT ENKS KFGA NAIL GVSL AVCK AGAV EKGV PLYR HIAD LAGN SEVI LPVP AFNV INGG SHAG NKLA MQEF MILP VGAA NFRE AMRI GAEV YHNL KNVI KEKY GKDA TNVG DEGG FAPN ILEN KEGL ELLK TAIG KAGY TDKV VIGM DVAA SEFF RSGK YDLD FKSP DDPS RYIS PDQL ADLY KSFI KDYP VVSI EDPF DQDD WGAW QKFT ASAG IQVV GDDL TVTN PKRI AKAV NEKS CNCL LLKV NQIG SVTE SLQA CKLA QANG WGVM VSHR SGET EDTF IADL VVGL CTGQ IKTG APCR SERL AKYN QLLR IEEE LGSK AKFA GRNF RNPL AK
Gene ID:2023
Uniprot:P06733
Cellular Location:Cell membrane, Cytoplasm, M line, Nucleus, myofibril, sarcomere
Calculated MW:36kDa/47kDa
Observed MW:47kDa
Synonyms:ENO1, ENO1L1, HEL-S-17, MPB1, NNE, PPH, enolase 1
Background:This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.
UniProt Protein Function:ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor; EC 4.2.1.11; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: cytoplasm; cytosol; extracellular space; M band; membrane; nucleus; phosphopyruvate hydratase complex; plasma membrane

Molecular Function:DNA binding; GTPase binding; magnesium ion binding; phosphopyruvate hydratase activity; protein binding; transcription corepressor activity; transcription factor activity

Biological Process: carbohydrate metabolic process; gluconeogenesis; glucose metabolic process; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; response to virus; transcription, DNA-dependent

NCBI Summary:This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
UniProt Code:P06733
NCBI GenInfo Identifier:119339
NCBI Gene ID:2023
NCBI Accession:P06733.2
UniProt Secondary Accession:P06733,P22712, Q16704, Q4TUS4, Q53FT9, Q53HR3, Q658M5 Q6GMP2, Q71V37, Q7Z3V6, Q8WU71, B2RD59,
UniProt Related Accession:P06733
Molecular Weight:36,928 Da
NCBI Full Name:Alpha-enolase
NCBI Synonym Full Names:enolase 1, (alpha)
NCBI Official Symbol:ENO1  
NCBI Official Synonym Symbols:NNE; PPH; MPB1; ENO1L1; HEL-S-17  
NCBI Protein Information:alpha-enolase; c-myc promoter-binding protein-1
UniProt Protein Name:Alpha-enolase
UniProt Synonym Protein Names:2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein
UniProt Gene Name:ENO1  
UniProt Entry Name:ENOA_HUMAN
Anti-ENO1 Antibody [KO Validated] (CAB1033)Western blot analysis of extracts of various cell lines, using ENO1 antibody (CAB1033) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-ENO1 Antibody [KO Validated] (CAB1033)Western blot analysis of extracts from normal (control) and ENO1 knockout (KO) HeLa cells, using ENO1 antibody (CAB1033) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-ENO1 Antibody [KO Validated] (CAB1033)Immunohistochemistry of paraffin-embedded human colon carcinoma using ENO1 antibody (CAB1033) at dilution of 1:100 (40x lens).
Anti-ENO1 Antibody [KO Validated] (CAB1033)Immunofluorescence analysis of NIH-3T3 cells using ENO1 antibody (CAB1033) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-ENO1 Antibody [KO Validated] (CAB1033)Immunofluorescence analysis of U-2 OS cells using ENO1 antibody (CAB1033) at dilution of 1:100. Blue: DAPI for nuclear staining.
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