Cardiovascular Antibodies

Anti-ENG Antibody (CAB5639)

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SKU:
CAB5639
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular

Description

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Antibody Name:Anti-ENG Antibody
Antibody SKU:CAB5639
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 26-306 of human ENG (NP_001108225.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:293T, HL-60, THP-1, HeLa, NCI-H460
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 26-306 of human ENG (NP_001108225.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ETVH CDLQ PVGP ERGE VTYT TSQV SKGC VAQA PNAI LEVH VLFL EFPT GPSQ LELT LQAS KQNG TWPR EVLL VLSV NSSV FLHL QALG IPLH LAYN SSLV TFQE PPGV NTTE LPSF PKTQ ILEW AAER GPIT SAAE LNDP QSIL LRLG QAQG SLSF CMLE ASQD MGRT LEWR PRTP ALVR GCHL EGVA GHKE AHIL RVLP GHSA GPRT VTVK VELS CAPG DLDA VLIL QGPP YVSW LIDA NHNM QIWT TGEY SFKI FPEK NIRG FKLP DTPQ GLLG EARM L
Gene ID:2022
Uniprot:P17813
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:67kDa/70kDa
Observed MW:71kDa
Synonyms:END, HHT1, ORW1, CD105, ENG, endoglin
Background:This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:ENG: Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Homodimer that forms an heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Interacts with TCTEX1D4. Interacts with ARRB2. Endoglin is restricted to endothelial cells in all tissues except bone marrow. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: cytoplasm; external side of plasma membrane; extracellular space; focal adhesion; nucleoplasm; receptor complex

Molecular Function:activin binding; galactose binding; glycosaminoglycan binding; protein binding; punt binding; transforming growth factor beta binding; transforming growth factor beta receptor, cytoplasmic mediator activity

Biological Process: artery morphogenesis; BMP signaling pathway; central nervous system vasculogenesis; heart looping; negative regulation of cell migration; negative regulation of protein amino acid autophosphorylation; patterning of blood vessels; positive regulation of BMP signaling pathway; positive regulation of protein amino acid phosphorylation; regulation of transcription, DNA-dependent; regulation of transforming growth factor beta receptor signaling pathway; smooth muscle development; vasculogenesis; venous blood vessel morphogenesis

Disease: Telangiectasia, Hereditary Hemorrhagic, Of Rendu, Osler, And Weber

NCBI Summary:This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]
UniProt Code:P17813
NCBI GenInfo Identifier:3041681
NCBI Gene ID:2022
NCBI Accession:P17813.2
UniProt Secondary Accession:P17813,Q14248, Q14926, Q5T9C0,
UniProt Related Accession:P17813
Molecular Weight:67,542 Da
NCBI Full Name:Endoglin
NCBI Synonym Full Names:endoglin
NCBI Official Symbol:ENG  
NCBI Official Synonym Symbols:END; HHT1; ORW1  
NCBI Protein Information:endoglin
UniProt Protein Name:Endoglin
UniProt Synonym Protein Names:CD_antigen: CD105
Protein Family:Endoglin
UniProt Gene Name:ENG  
UniProt Entry Name:EGLN_HUMAN
Anti-ENG Antibody (CAB5639)Western blot analysis of extracts of various cell lines, using ENG antibody (CAB5639) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
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