Cell Biology Antibodies 12
Anti-Endomucin Antibody (CAB8494)
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- SKU:
- CAB8494
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Endomucin Antibody |
| Antibody SKU: | CAB8494 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-135 of human EMCN (NP_057326.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | MCF7, NIH/3T3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-135 of human EMCN (NP_057326.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NSTG VLEA ANNS LVVT TTKP SITT PNTE SLQK NVVT PTTG TTPK GTIT NELL KMSL MSTA TFLT SKDE GLKA TTTD VRKN DSII SNVT VTSV TLPN AVST LQSS KPKT ETQS SIKT T |
| Gene ID: | 51705 |
| Uniprot: | Q9ULC0 |
| Cellular Location: | Cell membrane, Membrane, Secreted, Single-pass type I membrane protein, Single-pass type I membrane protein |
| Calculated MW: | 18kDa/26kDa/27kDa |
| Observed MW: | 35kDa |
| Synonyms: | EMCN, EMCN2, MUC14, endomucin |
| Background: |
| UniProt Protein Function: | EMCN: Endothelial sialomucin, also called endomucin or mucin- like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell adhesion; Membrane protein, integral Chromosomal Location of Human Ortholog: 4q24 Cellular Component: integral to membrane; plasma membrane; extracellular region |
| NCBI Summary: | EMCN is a mucin-like sialoglycoprotein that interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix (Kinoshita et al., 2001 [PubMed 11418125]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9ULC0 |
| NCBI GenInfo Identifier: | 229576885 |
| NCBI Gene ID: | 51705 |
| NCBI Accession: | NP_001153166.1 |
| UniProt Secondary Accession: | Q9ULC0,Q8NEY5, Q8WWE7, Q9NRM8, A8K716, B4E347, |
| UniProt Related Accession: | Q9ULC0 |
| Molecular Weight: | 27,452 Da |
| NCBI Full Name: | endomucin isoform 2 |
| NCBI Synonym Full Names: | endomucin |
| NCBI Official Symbol: | EMCN |
| NCBI Official Synonym Symbols: | EMCN2; MUC14 |
| NCBI Protein Information: | endomucin; MUC-14; mucin-14; endomucin-2; gastric cancer antigen Ga34 |
| UniProt Protein Name: | Endomucin |
| UniProt Synonym Protein Names: | Endomucin-2; Gastric cancer antigen Ga34; Mucin-14; MUC-14 |
| Protein Family: | Endomucin |
| UniProt Gene Name: | EMCN |
| UniProt Entry Name: | MUCEN_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EMCN antibody (CAB8494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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