Anti-ENDOGL1 / ENGL Antibody (CAB20881)

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SKU:
CAB20881
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Plant
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-ENDOGL1 / ENGL Antibody (CAB20881)
Antibody SKU:CAB20881
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant protein of Human ENDOGL1 / ENGL.
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HL-60, HUVEC, MCF7
Immunogen:Recombinant protein of Human ENDOGL1 / ENGL.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Mitochondrion inner membrane
Calculated MW:16kDa/26kDa/35kDa/41kDa
Observed MW:41kDa
Synonyms:EXOG, ENDOGL1, ENDOGL2, ENGL, ENGL-a, ENGL-b, ENGLA, ENGLB
Background:This gene encodes an endo/exonuclease with 5'-3' exonuclease activity. The encoded enzyme catalyzes the hydrolysis of ester linkages at the 5' end of a nucleic acid chain. This enzyme is localized to the mitochondria and may play a role in programmed cell death. Alternatively spliced transcript variants have been described. A pseudogene exists on chromosome 18.
Western blot analysis of extracts of various cell lines, using ENDOGL1/ENGL antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.Western blot analysis of extracts of various cell lines, using ENDOGL1/ENGL antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
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