Cell Biology Antibodies 1
Anti-Endo G Antibody (CAB0604)
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- SKU:
- CAB0604
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Endo G Antibody |
| Antibody SKU: | CAB0604 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 49-297 of human Endo G (NP_004426.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, MCF7, SW620, Mouse heart, Mouse kidney, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 49-297 of human Endo G (NP_004426.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AELP PVPG GPRG PGEL AKYG LPGL AQLK SRES YVLC YDPR TRGA LWVV EQLR PERL RGDG DRRE CDFR EDDS VHAY HRAT NADY RGSG FDRG HLAA AANH RWSQ KAMD DTFY LSNV APQV PHLN QNAW NNLE KYSR SLTR SYQN VYVC TGPL FLPR TEAD GKSY VKYQ VIGK NHVA VPTH FFKV LILE AAGG QIEL RTYV MPNA PVDE AIPL ERFL VPIE SIER ASGL LFVP NILA RAGS LKAI TAGS K |
| Gene ID: | 2021 |
| Uniprot: | Q14249 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 32kDa |
| Observed MW: | 33kDa |
| Synonyms: | ENDOG |
| Background: | The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. |
| UniProt Protein Function: | ENDOG: Cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. Homodimer. Belongs to the DNA/RNA non-specific endonuclease family. |
| UniProt Protein Details: | Protein type:EC 3.1.30.-; Ribonuclease; Deoxyribonuclease; Apoptosis; Mitochondrial Chromosomal Location of Human Ortholog: 9q34.1 Cellular Component: mitochondrion Molecular Function:protein binding; nuclease activity; nucleic acid binding; metal ion binding; endonuclease activity Biological Process: response to antibiotic; positive regulation of apoptosis; in utero embryonic development; DNA fragmentation during apoptosis; DNA recombination |
| NCBI Summary: | The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q14249 |
| NCBI GenInfo Identifier: | 24638471 |
| NCBI Gene ID: | 2021 |
| NCBI Accession: | Q14249.3 |
| UniProt Secondary Accession: | Q14249,Q5T281, Q9BSP2, |
| UniProt Related Accession: | Q14249 |
| Molecular Weight: | 32,620 Da |
| NCBI Full Name: | Endonuclease G, mitochondrial |
| NCBI Synonym Full Names: | endonuclease G |
| NCBI Official Symbol: | ENDOG |
| NCBI Protein Information: | endonuclease G, mitochondrial; endo G; mitochondrial endonuclease G |
| UniProt Protein Name: | Endonuclease G, mitochondrial |
| Protein Family: | Endoglin |
| UniProt Gene Name: | ENDOG |
| UniProt Entry Name: | NUCG_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Endo G antibody (CAB0604) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using Endo G antibody (CAB0604) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using Endo G antibody (CAB0604) at dilution of 1:100 (40x lens). |
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