Cell Biology Antibodies 15

Anti-ENAH Antibody (CAB17932)

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SKU:
CAB17932
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ENAH Antibody
Antibody SKU:CAB17932
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 412-591 of human ENAH (NP_001008493.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, C6, rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 412-591 of human ENAH (NP_001008493.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FPSG GNAI GVNS ASSK TDTG RGNG PLPL GGSG LMEE MSAL LARR RRIA EKGS TIET EQKE DKGE DSEP VTSK ASST STPE PTRK PWER TNTM NGSK SPVI SRRD SPRK NQIV FDNR SYDS LHRP KSTP LSQP SANG VQTE GLDY DRLK QDIL DEMR KELT KLKE ELID AIRQ ELSK SNTA
Gene ID:55740
Uniprot:Q8N8S7
Cellular Location:
Calculated MW:
Observed MW:110kDa
Anti-ENAH Antibody (CAB17932)Western blot - ENAH Polyclonal Antibody (CAB17932)
Synonyms:ENA, MENA, NDPP1, ENAH
Background:
UniProt Protein Function:Mena: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes. Homotetramer. Interacts with APBB1IP, APBB1, PFN1 and ROBO4. Isoforms, containing the polyproline-rich regions with PPLP motifs, bind the WW domain of APBB1IP. Isoforms, containing the PPSY motif, bind, in vitro, to the WW2 and WW3 domains of NEDD4 and to the WW1 domain of YAP1. Binds the SH3 domain of BAIAP2-alpha but only after the autoinhibitory region of BAIAP2-alpha has been blocked by interaction with CDC42. Interacts, via the EVH1/WH1 domain, with the Pro-rich domains from VCL, ZYX and Listeria monocytogenes actA and with TES (via LIM domains). The TES LIM domain and the Pro-rich domains from VCL or ZYX compete for the same binding site. Interaction with ZYX is important for targeting ENAH to focal adhesions and enhances production of actin-rich structures at the apical surface of cells. Interacts, through the Pro-rich region, with the C-terminal SH3 domain of DNMPB. Binds GPHN. Interacts with FAT1 (via EVH1 domains). Heterotrimer with TES and ACTL7A. Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions. Belongs to the Ena/VASP family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q42.12

Cellular Component: cell junction; cytoplasm; cytosol; filopodium; focal adhesion; lamellipodium; plasma membrane; stress fiber; synapse

Molecular Function:actin binding; protein binding; SH3 domain binding; WW domain binding

Biological Process: actin polymerization and/or depolymerization; axon guidance; intracellular transport; neural tube closure; T cell receptor signaling pathway

UniProt Code:Q8N8S7
NCBI GenInfo Identifier:48428086
NCBI Gene ID:55740
NCBI Accession:Q8N8S7.2
UniProt Secondary Accession:Q8N8S7,Q502W5, Q5T5M7, Q5VTQ9, Q5VTR0, Q9NVF3, Q9UFB8 D0PQI2,
UniProt Related Accession:Q8N8S7
Molecular Weight:60,349 Da
NCBI Full Name:Protein enabled homolog
NCBI Synonym Full Names:enabled homolog (Drosophila)
NCBI Official Symbol:ENAH  
NCBI Official Synonym Symbols:ENA; MENA; NDPP1  
NCBI Protein Information:protein enabled homolog
UniProt Protein Name:Protein enabled homolog
Protein Family:Menaquinone reductase
UniProt Gene Name:ENAH  
UniProt Entry Name:ENAH_HUMAN
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