Name: Anti-EMR2 Antibody (CAB14278)
Brand: Cell Biology Antibodies 4
SKU: CAB14278
Availability: OutOfStock

Antibody Name:	Anti-EMR2 Antibody
Antibody SKU:	CAB14278
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 300-500 of human EMR2 (NP_038475.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	K-562, LO2, A-549, HeLa, Mouse thymus, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 300-500 of human EMR2 (NP_038475.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TIQS ILQA LDEL LEAP GDLE TLPR LQQH CVAS HLLD GLED VLRG LSKN LSNG LLNF SYPA GTEL SLEV QKQV DRSV TLRQ NQAV MQLD WNQA QKSG DPGP SVVG LVSI PGMG KLLA EAPL VLEP EKQM LLHE THQG LLQD GSPI LLSD VISA FLSN NDTQ NLSS PVTF TFSH RSVI PRQK VLCV FWEH GQNG CGHW ATTG C
Gene ID:	30817
Uniprot:	Q9UHX3
Cellular Location:	Cell membrane, Cell projection, Multi-pass membrane protein, ruffle membrane
Calculated MW:	74kDa/80kDa/84kDa/85kDa/89kDa/90kDa
Observed MW:	100kDa

Synonyms:

ADGRE2, CD312, EMR2, VBU

Background:

This gene encodes a member of the class B seven-span transmembrane (TM7) subfamily of G-protein coupled receptors. These proteins are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a TM7 domain via a mucin-like spacer domain. The encoded protein is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with chondroitin sulfate chains. This gene is situated in a cluster of related genes on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	EMR2: Cell surface receptor that binds to the chondroitin sulfate moiety of glycosaminoglycan chains and promotes cell attachment. Promotes granulocyte chemotaxis, degranulation and adhesion. In macrophages, promotes the release of inflammatory cytokines, including IL8 and TNF. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; GPCR, family 2; Membrane protein, multi-pass; Receptor, GPCR Chromosomal Location of Human Ortholog: 19p13.1 Cellular Component: plasma membrane; integral to membrane Molecular Function:G-protein coupled receptor activity; calcium ion binding Biological Process: G-protein coupled receptor protein signaling pathway; cell migration; inflammatory response; cell adhesion
NCBI Summary:	This gene encodes a member of the class B seven-span transmembrane (TM7) subfamily of G-protein coupled receptors. These proteins are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a TM7 domain via a mucin-like spacer domain. The encoded protein is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with chondroitin sulfate chains. This gene is situated in a cluster of related genes on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
UniProt Code:	Q9UHX3
NCBI GenInfo Identifier:	108935835
NCBI Gene ID:	30817
NCBI Accession:	Q9UHX3.2
UniProt Secondary Accession:	Q9UHX3,Q8NG96, Q9Y4B1, B4DQ96, E7ESD7, E9PBR1, E9PEL6 E9PFQ5, E9PG91,
UniProt Related Accession:	Q9UHX3
Molecular Weight:	90,472 Da
NCBI Full Name:	EGF-like module-containing mucin-like hormone receptor-like 2
NCBI Synonym Full Names:	egf-like module containing, mucin-like, hormone receptor-like 2
NCBI Official Symbol:	EMR2
NCBI Official Synonym Symbols:	CD312
NCBI Protein Information:	EGF-like module-containing mucin-like hormone receptor-like 2
UniProt Protein Name:	EGF-like module-containing mucin-like hormone receptor-like 2
UniProt Synonym Protein Names:	EGF-like module receptor 2
UniProt Gene Name:	EMR2
UniProt Entry Name:	EMR2_HUMAN

Western blot analysis of extracts of various cell lines, using EMR2 Rabbit pAb (CAB14278) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

EMR2 Antibody (PACO02809)

EMR2 Antibody (PACO04043)

EMR2 Antibody (PACO06909)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)