Antibody Name:	Anti-EMR1 Antibody
Antibody SKU:	CAB1256
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-290 of human EMR1 (NP_001243182.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-290 of human EMR1 (NP_001243182.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HIRP TRKP NTKG NNCR DSTL CPAY ATCT NTVD SYYC ACKQ GFLS SNGQ NHFK DPGV RCKD IDEC SQSP QPCG PNSS CKNL SGRY KCSC LDGF SSPT GNDW VPGK PGNF SCTD INEC LTSS VCPE HSDC VNSM GSYS CSCQ VGFI SRNS TCED VDEC ADPR ACPE HATC NNTV GNYS CFCN PGFE SSSG HLSF QGLK ASCE DIDE CTEM CPIN STCT NTPG SYFC TCHP GFAP SNGQ LNFT DQGV ECRD IDEC RQDP STCG PNSI CTNA LG
Gene ID:	2015
Uniprot:	Q14246
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	78kDa/82kDa/90kDa/95kDa/97kDa
Observed MW:	160KDa

Synonyms:

ADGRE1, EMR1, TM7LN3, F4/80

Background:

This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
NCBI Summary:	This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Code:	Q14246
NCBI GenInfo Identifier:	372266078
NCBI Gene ID:	2015
NCBI Accession:	NP_001243181.1
UniProt Secondary Accession:	Q14246,Q2I7G5, Q6ZMN0, Q8NGA7, A6NHV2, B7Z486, B7Z489 E7EPX9, E9PD45, H9KV79,
UniProt Related Accession:	Q14246
Molecular Weight:	102 kDa
NCBI Full Name:	adhesion G protein-coupled receptor E1 isoform 2
NCBI Synonym Full Names:	adhesion G protein-coupled receptor E1
NCBI Official Symbol:	ADGRE1
NCBI Official Synonym Symbols:	EMR1; TM7LN3
NCBI Protein Information:	adhesion G protein-coupled receptor E1
UniProt Protein Name:	Adhesion G protein-coupled receptor E1
UniProt Synonym Protein Names:	EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor
UniProt Gene Name:	ADGRE1

	Western blot analysis of extracts of Mouse lung, using EMR1 antibody (CAB1256) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of Raw264 7 cells using EMR1 Rabbit pAb (CAB1256) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of THP-1 cells using EMR1 Rabbit pAb (CAB1256) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.