Cell Biology Antibodies 6
Anti-EML4 Antibody (CAB16117)
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- SKU:
- CAB16117
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-EML4 Antibody |
| Antibody SKU: | CAB16117 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 360-560 of human EML4 (NP_061936.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 360-560 of human EML4 (NP_061936.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GTFE RGVG CLDF SKAD SGVH LCVI DDSN EHML TVWD WQKK AKGA EIKT TNEV VLAV EFHP TDAN TIIT CGKS HIFF WTWS GNSL TRKQ GIFG KYEK PKFV QCLA FLGN GDVL TGDS GGVM LIWS KTTV EPTP GKGP KGVY QISK QIKA HDGS VFTL CQMR NGML LTGG GKDR KIIL WDHD LNPE REIE VPDQ YGTI RAVA E |
| Gene ID: | 27436 |
| Uniprot: | Q9HC35 |
| Cellular Location: | Cytoplasm, cytoskeleton |
| Calculated MW: | 102kDa/108kDa |
| Observed MW: | 120kDa |
| Synonyms: | EML4, C2orf2, ELP120, EMAP-4, EMAPL4, ROPP120 |
| Background: | This gene is a member of the echinoderm microtubule associated protein-like family. The encoded WD-repeat protein may be involved in microtubule formation. Abnormal fusion of parts of this gene with portions of the anaplastic lymphoma receptor tyrosine kinase gene, which generates EML4-ALK fusion transcripts, is one of the primary mutations associated with non-small cell lung cancer. Alternative splicing of this gene results in two transcript variants. |
| UniProt Protein Function: | EML4: a microtubule-associated protein. A WD-repeat protein associated with the mitotic spindle apparatus. May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic. Strongly overexpressed during mitosis. |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 2p21 Cellular Component: membrane; microtubule cytoskeleton Molecular Function:microtubule binding Biological Process: microtubule cytoskeleton organization and biogenesis |
| NCBI Summary: | This gene is a member of the echinoderm microtubule associated protein-like family. The encoded WD-repeat protein may be involved in microtubule formation. Abnormal fusion of parts of this gene with portions of the anaplastic lymphoma receptor tyrosine kinase gene, which generates EML4-ALK fusion transcripts, is one of the primary mutations associated with non-small cell lung cancer. Alternative splicing of this gene results in two transcript variants. [provided by RefSeq, Jan 2015] |
| UniProt Code: | Q9HC35 |
| NCBI GenInfo Identifier: | 14250486 |
| NCBI Gene ID: | 27436 |
| NCBI Accession: | BC008685 |
| UniProt Secondary Accession: | Q9HC35,Q3SWW0, Q53R29, Q53TW8, Q6PJ45, Q9NV40, A6H8Y6 B2RBK3, B2RTW7, B5MCW9, |
| UniProt Related Accession: | Q9HC35 |
| Molecular Weight: | 102,444 Da |
| NCBI Full Name: | Homo sapiens echinoderm microtubule associated protein like 4, mRNA |
| NCBI Synonym Full Names: | echinoderm microtubule associated protein like 4 |
| NCBI Official Symbol: | EML4 |
| NCBI Official Synonym Symbols: | C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120 |
| NCBI Protein Information: | echinoderm microtubule-associated protein-like 4 |
| UniProt Protein Name: | Echinoderm microtubule-associated protein-like 4 |
| UniProt Synonym Protein Names: | Restrictedly overexpressed proliferation-associated protein; Ropp 120 |
| Protein Family: | Echinoderm microtubule-associated protein |
| UniProt Gene Name: | EML4 |
| UniProt Entry Name: | EMAL4_HUMAN |
 | Western blot analysis of extracts of HepG2 cells, using EML4 Rabbit pAb (CAB16117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded Mouse kidney using EML4 Rabbit pAb (CAB16117) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat kidney using EML4 Rabbit pAb (CAB16117) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human liver cancer using EML4 Rabbit pAb (CAB16117) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of L929 cells using EML4 antibody (CAB16117) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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