Name: Anti-EMD Antibody (CAB1132)
Brand: Cell Biology Antibodies 2
SKU: CAB1132
Availability: OutOfStock

Antibody Name:	Anti-EMD Antibody
Antibody SKU:	CAB1132
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human EMD (NP_000108.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	HeLa, Jurkat, PC-3, U-251MG, Y79

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human EMD (NP_000108.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDNY ADLS DTEL TTLL RRYN IPHG PVVG STRR LYEK KIFE YETQ RRRL SPPS SSAA SSYS FSDL NSTR GDAD MYDL PKKE DALL YQSK GYND DYYE ESYF TTRT YGEP ESAG PSRA VRQS VTSF PDAD AFHH QVHD DDLL SSSE EECK DRER PMYG RDSA YQSI THYR PVSA SRSS LDLS YYPT SSST SFMS SSSS SSSW LTRR AIRP ENRA PGAG LGQD
Gene ID:	2010
Uniprot:	P50402
Cellular Location:	Nucleoplasmic side, Nucleus inner membrane, Nucleus outer membrane, Single-pass membrane protein
Calculated MW:	28kDa
Observed MW:	35kDa

Synonyms:	EMD, EDMD, LEMD5, STA, emerin
Background:	Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

UniProt Protein Function:	emerin: a single pass membrane protein that spans the inner nuclear membrane. A serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It interacts with lamins A and C, and mediates membrane anchorage to the cytoskeleton. Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through an exportin 1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. Colocalized with BAF at the central region of the assembling nuclear rim, near spindle-attachment sites. Hyper-phosphorylated on tyrosine in cells overexpressing HER2. Directly phosphorylated by Src and Abl. Phosphorylated by Src at Y59, Y74 and Y95. May function as a downstream effector and signal integrator for tyrosine kinase signaling pathways at the nuclear envelope. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.
UniProt Protein Details:	Protein type:Cytoskeletal; Membrane protein, integral Chromosomal Location of Human Ortholog: Xq28 Cellular Component: microtubule; nuclear outer membrane; nuclear membrane; membrane; endoplasmic reticulum; integral to membrane; nuclear inner membrane; nuclear envelope Molecular Function:protein binding; beta-tubulin binding; actin binding Biological Process: positive regulation of protein export from nucleus; mitotic nuclear envelope reassembly; muscle development; muscle contraction; negative regulation of fibroblast proliferation; mitotic nuclear envelope disassembly; mitotic cell cycle Disease: Emery-dreifuss Muscular Dystrophy 1, X-linked
NCBI Summary:	Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P50402
NCBI GenInfo Identifier:	1706639
NCBI Gene ID:	2010
NCBI Accession:	P50402.1
UniProt Secondary Accession:	P50402,Q6FI02,
UniProt Related Accession:	P50402
Molecular Weight:
NCBI Full Name:	Emerin
NCBI Synonym Full Names:	emerin
NCBI Official Symbol:	EMD
NCBI Official Synonym Symbols:	STA; EDMD; LEMD5
NCBI Protein Information:	emerin; LEM domain containing 5
UniProt Protein Name:	Emerin
Protein Family:	Emerin
UniProt Gene Name:	EMD
UniProt Entry Name:	EMD_HUMAN

	Western blot analysis of extracts of various cell lines, using EMD antibody (CAB1132) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human uterus using EMD antibody (CAB1132) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using EMD antibody (CAB1132). Blue: DAPI for nuclear staining.
	Confocal immunofluorescence analysis of U-2 OS cells using EMD Polyclonal Antibody (CAB1132) at dilution of 1:100. Blue: DAPI for nuclear staining.