Name: Anti-EMCN Antibody (CAB14131)
Brand: Cell Biology Antibodies 4
SKU: CAB14131
Availability: OutOfStock

Antibody Name:	Anti-EMCN Antibody
Antibody SKU:	CAB14131
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-135 of human EMCN (NP_057326.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, SGC-7901, Mouse brain, Rat spinal cord

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-135 of human EMCN (NP_057326.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NSTG VLEA ANNS LVVT TTKP SITT PNTE SLQK NVVT PTTG TTPK GTIT NELL KMSL MSTA TFLT SKDE GLKA TTTD VRKN DSII SNVT VTSV TLPN AVST LQSS KPKT ETQS SIKT T
Gene ID:	51705
Uniprot:	Q9ULC0
Cellular Location:	Cell membrane, Membrane, Secreted, Single-pass type I membrane protein, Single-pass type I membrane protein
Calculated MW:	18kDa/26kDa/27kDa
Observed MW:	36kDa

Synonyms:	EMCN, EMCN2, MUC14, endomucin
Background:

UniProt Protein Function:	EMCN: Endothelial sialomucin, also called endomucin or mucin- like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell adhesion; Membrane protein, integral Chromosomal Location of Human Ortholog: 4q24 Cellular Component: integral to membrane; plasma membrane; extracellular region
NCBI Summary:	EMCN is a mucin-like sialoglycoprotein that interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix (Kinoshita et al., 2001 [PubMed 11418125]).[supplied by OMIM, Mar 2008]
UniProt Code:	Q9ULC0
NCBI GenInfo Identifier:	229576885
NCBI Gene ID:	51705
NCBI Accession:	NP_001153166.1
UniProt Secondary Accession:	Q9ULC0,Q8NEY5, Q8WWE7, Q9NRM8, A8K716, B4E347,
UniProt Related Accession:	Q9ULC0
Molecular Weight:	27,452 Da
NCBI Full Name:	endomucin isoform 2
NCBI Synonym Full Names:	endomucin
NCBI Official Symbol:	EMCN
NCBI Official Synonym Symbols:	EMCN2; MUC14
NCBI Protein Information:	endomucin; MUC-14; mucin-14; endomucin-2; gastric cancer antigen Ga34
UniProt Protein Name:	Endomucin
UniProt Synonym Protein Names:	Endomucin-2; Gastric cancer antigen Ga34; Mucin-14; MUC-14
Protein Family:	Endomucin
UniProt Gene Name:	EMCN
UniProt Entry Name:	MUCEN_HUMAN

Western blot analysis of extracts of various cell lines, using EMCN antibody (CAB14131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

Anti-Endomucin Antibody (CAB8494)

EMCN Antibody (PACO04569)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)