Epigenetics & Nuclear Signaling Antibodies 4
Anti-ELF2 Antibody (CAB7487)
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- SKU:
- CAB7487
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-ELF2 Antibody |
| Antibody SKU: | CAB7487 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human ELF2 (NP_006865.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HL-60, A-549, MCF7, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human ELF2 (NP_006865.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MATS LHEG PTNQ LDLL IRAV EASV HSSN AHCT DKTI EAAE ALLH MESP TCLR DSRS PVEV FVPP CVST PEFI HAAM RPDV ITET VVEV STEE SEPM DTSP IPTS PDSH EPMK KKKV GRKP KTQQ SPIS NGSP ELGI KKKP |
| Gene ID: | 1998 |
| Uniprot: | Q15723 |
| Cellular Location: | Nucleus |
| Calculated MW: | 54kDa/56kDa/57kDa/62kDa/63kDa |
| Observed MW: | 64kDa |
| Synonyms: | ELF2, EU32, NERF, NERF-1A, NERF-1B, NERF-1a, b, NERF-2 |
| Background: |
| UniProt Protein Function: | Elf-2: Isoform 1 transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter. Belongs to the ETS family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 4q28 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:protein binding; sequence-specific DNA binding; transcription factor activity Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; cell differentiation; negative regulation of transcription, DNA-dependent |
| UniProt Code: | Q15723 |
| NCBI GenInfo Identifier: | 68052029 |
| NCBI Gene ID: | 1998 |
| NCBI Accession: | Q15723.2 |
| UniProt Secondary Accession: | Q15723,Q15724, Q15725, Q6P1K5, E9PCX3, |
| UniProt Related Accession: | Q15723 |
| Molecular Weight: | 593 |
| NCBI Full Name: | ETS-related transcription factor Elf-2 |
| NCBI Synonym Full Names: | E74-like factor 2 (ets domain transcription factor) |
| NCBI Official Symbol: | ELF2 |
| NCBI Official Synonym Symbols: | EU32; NERF; NERF-2; NERF-1A; NERF-1B; NERF-1a,b |
| NCBI Protein Information: | ETS-related transcription factor Elf-2; ELF2; new Ets-related factor; ets family transcription factor ELF2C |
| UniProt Protein Name: | ETS-related transcription factor Elf-2 |
| UniProt Synonym Protein Names: | E74-like factor 2; New ETS-related factor |
| Protein Family: | ETS-related transcription factor |
| UniProt Gene Name: | ELF2 |
| UniProt Entry Name: | ELF2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ELF2 antibody (CAB7487) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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