Antibody Name:	Anti-EIF6 Antibody
Antibody SKU:	CAB1818
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human EIF6 (NP_852133.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	U-251MG, HeLa, 293T, HepG2, K-562

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human EIF6 (NP_852133.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAVR ASFE NNCE IGCF AKLT NTYC LVAI GGSE NFYS VFEG ELSD TIPV VHAS IAGC RIIG RMCV GNRH GLLV PNNT TDQE LQHI RNSL PDTV QIRR VEER LSAL GNVT TCND YVAL VHPD LDRE TEEI LADV LKVE VFRQ TVAD QVLV GSYC VFSN QGGL VHPK TSIE DQDE LSSL LQVP LVAG TVNR GSEV IAAG MVVN DWCA FCGL DTTS TELS VVES VFKL NEAQ PSTI ATSM RDSL IDSL T
Gene ID:	3692
Uniprot:	P56537
Cellular Location:	Cytoplasm, Nucleus, nucleolus
Calculated MW:	23kDa/26kDa
Observed MW:	27kDa

Synonyms:

EIF6, CAB, EIF3A, ITGB4BP, b(2)gcn, eIF-6, p27(BBP), p27BBP

Background:

Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene.

UniProt Protein Function:	EIF6: eukaryotic translation initiation factor 6. Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex.
UniProt Protein Details:	Protein type:Translation; Translation initiation; Nucleolus Chromosomal Location of Human Ortholog: 20q12 Cellular Component: nucleoplasm; cytoplasm; nucleolus; lamin filament; nucleus Molecular Function:protein binding; translation initiation factor activity; ribosome binding; ribosomal large subunit binding Biological Process: translational initiation; ribosomal large subunit biogenesis and assembly; mature ribosome assembly; ribosome export from nucleus
NCBI Summary:	Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
UniProt Code:	P56537
NCBI GenInfo Identifier:	3122258
NCBI Gene ID:	3692
NCBI Accession:	P56537.1
UniProt Related Accession:	P56537
Molecular Weight:
NCBI Full Name:	Eukaryotic translation initiation factor 6
NCBI Synonym Full Names:	eukaryotic translation initiation factor 6
NCBI Official Symbol:	EIF6
NCBI Official Synonym Symbols:	CAB; EIF3A; eIF-6; p27BBP; ITGB4BP; b(2)gcn; p27(BBP)
NCBI Protein Information:	eukaryotic translation initiation factor 6
UniProt Protein Name:	Eukaryotic translation initiation factor 6
UniProt Synonym Protein Names:	B(2)GCN homolog; B4 integrin interactor; CAB; p27(BBP)
UniProt Gene Name:	EIF6
UniProt Entry Name:	IF6_HUMAN

	Western blot analysis of extracts of various cell lines, using EIF6 antibody (CAB1818) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of MCF-7 cells using EIF6 antibody (CAB1818). Blue: DAPI for nuclear staining.
	Confocal immunofluorescence analysis of Hela cells using EIF6 antibody (CAB1818) at dilution of 1:50. Blue: DAPI for nuclear staining.
	Confocal immunofluorescence analysis of U-2OS cells using EIF6 antibody (CAB1818) at dilution of 1:50. Blue: DAPI for nuclear staining.