Antibody Name:	Anti-EIF5B Antibody
Antibody SKU:	CAB15123
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human EIF5B (NP_056988.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	DU145, HeLa, Jurkat, LO2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human EIF5B (NP_056988.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGKK QKNK SEDS TKDD IDLD ALAA EIEG AGAA KEQE PQKS KGKK KKEK KKQD FDED DILK ELEE LSLE AQGI KADR ETVA VKPT ENNE EEFT SKDK KKKG QKGK KQSF DDND SEEL EDKD SKSK KTAK PKVE MYSG SDDD DDFN KLPK KAKG KAQK SNKK WDGS EEDE DNSK KIKE RSRI NSSG ESGD ESDE FLQS RKGQ KKNQ KNKP GPNI ESGN EDDD ASFK IKTV AQKK AEKK ERER KKRD EEKA KLRK LKEK EELE TGKK DQSK QKES Q
Gene ID:	9669
Uniprot:	O60841
Cellular Location:	Cytoplasm
Calculated MW:	138kDa
Observed MW:	179kDa

Synonyms:

EIF5B, IF2

Background:

Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.

UniProt Protein Function:	EIF5B: Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2. Belongs to the IF-2 family.
UniProt Protein Details:	Protein type:Translation initiation; Translation Chromosomal Location of Human Ortholog: 2q11.2 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:GTPase activity; protein binding; GTP binding; translation initiation factor activity Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression; regulation of translational initiation
NCBI Summary:	Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008]
UniProt Code:	O60841
NCBI GenInfo Identifier:	269849752
NCBI Gene ID:	9669
NCBI Accession:	O60841.4
UniProt Secondary Accession:	O60841,O95805, Q53RV7, Q53SI8, Q9UF81, Q9UMN7,
UniProt Related Accession:	O60841
Molecular Weight:	1220
NCBI Full Name:	Eukaryotic translation initiation factor 5B
NCBI Synonym Full Names:	eukaryotic translation initiation factor 5B
NCBI Official Symbol:	EIF5B
NCBI Official Synonym Symbols:	IF2
NCBI Protein Information:	eukaryotic translation initiation factor 5B; eIF-5B; translation initiation factor IF2; translation initiation factor IF-2
UniProt Protein Name:	Eukaryotic translation initiation factor 5B
UniProt Synonym Protein Names:	Translation initiation factor IF-2
Protein Family:	Eukaryotic translation initiation factor
UniProt Gene Name:	EIF5B
UniProt Entry Name:	IF2P_HUMAN

	Western blot analysis of extracts of various cell lines, using EIF5B antibody (CAB15123). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunofluorescence analysis of C6 cells using EIF5B antibody (CAB15123) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using EIF5B antibody (CAB15123) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using EIF5B antibody (CAB15123) at dilution of 1:100. Blue: DAPI for nuclear staining.