Name: Anti-EIF4G3 Antibody (CAB14621)
Brand: Metabolism Antibodies 1
SKU: CAB14621
Availability: OutOfStock

Antibody Name:	Anti-EIF4G3 Antibody
Antibody SKU:	CAB14621
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1200 of human EIF4G3 (NP_003751.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	LO2, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1200 of human EIF4G3 (NP_003751.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HKEA KIEE QEEQ RKVQ QLMT KEKR RPGV QRVD EGGW NTVQ GAKN SRVL DPSK FLKI TKPT IDEK IQLV PKAQ LGSW GKGS SGGA KASE TDAL RSSA SSLN RFSA LQPP APSG STPS TPVE FDSR RTLT SRGS MGRE KNDK PLPS ATAR PNTF MRGG SSKD LLDN QSQE EQRR EMLE TVKQ LTGG VDVE RNST EAER NKTR E
Gene ID:	8672
Uniprot:	O43432
Cellular Location:
Calculated MW:	55kDa/146kDa/176kDa/177kDa
Observed MW:	250kDa

Synonyms:

EIF4G3, eIF-4G 3, eIF4G 3, eIF4GII, eIF-4G3, eIF4G3

Background:

The protein encoded by this gene is thought to be part of the eIF4F protein complex, which is involved in mRNA cap recognition and transport of mRNAs to the ribosome. Interestingly, a microRNA (miR-520c-3p) has been found that negatively regulates synthesis of the encoded protein, and this leads to a global decrease in protein translation and cell proliferation. Therefore, this protein is a key component of the anti-tumor activity of miR-520c-3p.

UniProt Protein Function:	EIF4G3: Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1. Interacts with EIF4A, EIF4E, eIF3 and PABPC1. Part of a complex with EIF4E. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4G1/EIF4G3 interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate eIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIFG3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Belongs to the eIF4G family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding; Translation; Translation initiation Chromosomal Location of Human Ortholog: 1p36.12 Cellular Component: eukaryotic translation initiation factor 4F complex; cytosol Molecular Function:translation factor activity, nucleic acid binding; translation initiation factor activity; RNA cap binding Biological Process: viral reproduction; cytokine and chemokine mediated signaling pathway; translational initiation; regulation of translational initiation
UniProt Code:	O43432
NCBI GenInfo Identifier:	223460878
NCBI Gene ID:	8672
NCBI Accession:	AAI36644.1
UniProt Secondary Accession:	O43432,Q15597, Q5SWC3, Q8NEN1, B9EGQ7,
UniProt Related Accession:	O43432
Molecular Weight:	176,652 Da
NCBI Full Name:	EIF4G3 protein
NCBI Synonym Full Names:	eukaryotic translation initiation factor 4 gamma, 3
NCBI Official Symbol:	EIF4G3
NCBI Official Synonym Symbols:	eIF4G 3; eIF4GII; eIF-4G 3
NCBI Protein Information:	eukaryotic translation initiation factor 4 gamma 3; eIF-4-gamma 3; eIF-4-gamma II
UniProt Protein Name:	Eukaryotic translation initiation factor 4 gamma 3
UniProt Synonym Protein Names:	eIF-4-gamma II
Protein Family:	Eukaryotic translation initiation factor
UniProt Gene Name:	EIF4G3
UniProt Entry Name:	IF4G3_HUMAN

Western blot analysis of extracts of various cell lines, using EIF4G3 antibody (CAB14621) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

EIF4G3 Antibody (PACO09059)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)