Antibody Name:	Anti-EIF4EBP1 Antibody [KO Validated]
Antibody SKU:	CAB1248
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human EIF4EBP1 (NP_004086.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HeLa, SH-SY5Y, NIH/3T3, Mouse fat, Mouse heart

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human EIF4EBP1 (NP_004086.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TRII YDRK FLME CRNS PVTK TPPR DLPT IPGV TSPS SDEP PMEA SQSH LRNS PEDK RAGG EESQ FEMD I
Gene ID:	1978
Uniprot:	Q13541
Cellular Location:
Calculated MW:	12kDa
Observed MW:	17kDa

Synonyms:

4E-BP1, 4EBP1, BP-1, PHAS-I, EIF4EBP1

Background:

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

UniProt Protein Function:	4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway.
UniProt Protein Details:	Protein type:Translation; Translation initiation Chromosomal Location of Human Ortholog: 8p12 Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol Molecular Function:protein binding; translation repressor activity; eukaryotic initiation factor 4E binding Biological Process: negative regulation of translational initiation; TOR signaling pathway; response to ethanol; cellular protein metabolic process; translation; translational initiation; insulin receptor signaling pathway; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; G1/S transition of mitotic cell cycle; lung development
NCBI Summary:	This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13541
NCBI GenInfo Identifier:	34921508
NCBI Gene ID:	1978
NCBI Accession:	Q13541.3
UniProt Secondary Accession:	Q13541,Q6IBN3, B2R502, D3DSW8,
UniProt Related Accession:	Q13541
Molecular Weight:	118
NCBI Full Name:	Eukaryotic translation initiation factor 4E-binding protein 1
NCBI Synonym Full Names:	eukaryotic translation initiation factor 4E binding protein 1
NCBI Official Symbol:	EIF4EBP1
NCBI Official Synonym Symbols:	BP-1; 4EBP1; 4E-BP1; PHAS-I
NCBI Protein Information:	eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1
UniProt Protein Name:	Eukaryotic translation initiation factor 4E-binding protein 1
UniProt Synonym Protein Names:	Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Protein Family:	Eukaryotic translation initiation factor 4E-binding protein
UniProt Gene Name:	EIF4EBP1
UniProt Entry Name:	4EBP1_HUMAN

	Western blot analysis of extracts of various cell lines, using EIF4EBP1 antibody (CAB1248) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Western blot analysis of extracts from normal (control) and EIF4EBP1 knockout (KO) HeLa cells, using EIF4EBP1 antibody (CAB1248) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of NIH/3T3 cells using EIF4EBP1 antibody (CAB1248) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-EIF4EBP1 Antibody (CAB16667)	4E-BP1 Colorimetric Cell-Based ELISA Kit
Anti-Phospho-EIF4EBP1-T37/46 Antibody (CABP0030)	4E-BP1 (Phospho-Ser64) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-EIF4EBP1-T70 Antibody (CABP0031)	4E-BP1 (Phospho-Thr36) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-EIF4EBP1-S65 Antibody (CABP0032)	4E-BP1 (Phospho-Thr45) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-EIF4EBP1-T45 Antibody (CABP0219)