Metabolism Antibodies 1
Anti-EIF4B Antibody (CAB13300)
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- SKU:
- CAB13300
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-EIF4B Antibody |
| Antibody SKU: | CAB13300 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 352-611 of human EIF4B (NP_001408.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | A-549, HeLa, 293T, MCF7, 22Rv1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 352-611 of human EIF4B (NP_001408.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SQST RAAS IFGG AKPV DTAA RERE VEER LQKE QEKL QRQL DEPK LERR PRER HPSW RSEE TQER ERSR TGSE SSQT GTST TSSR NARR RESE KSLE NETL NKEE DCHS PTSK PPKP DQPL KVMP APPP KENA WVKR SSNP PARS QSSD TEQQ SPTS GGGK VAPA QPSE EGPG RKDE NKVD GMNA PKGQ TGNS SRGP GDGG NRDH WKES DRKD GKKD QDSR SAPE PKKP EENP ASKF SSAS KYAA LSVD GEDE NEGE DYAE |
| Gene ID: | 1975 |
| Uniprot: | P23588 |
| Cellular Location: | |
| Calculated MW: | 64kDa/69kDa |
| Observed MW: | 80kDa |
| Synonyms: | EIF4B, EIF-4B, PRO1843 |
| Background: |
| UniProt Protein Function: | EIF4B: eukaryotic translation initiation factor 4B. Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. |
| UniProt Protein Details: | Protein type:RNA-binding; Translation initiation; Translation Chromosomal Location of Human Ortholog: 12q13.13 Cellular Component: cytosol; eukaryotic translation initiation factor 4F complex; polysome Molecular Function:helicase activity; protein binding; ribosomal small subunit binding; RNA binding; RNA strand annealing activity; RNA strand-exchange activity Biological Process: formation of translation preinitiation complex; poly(A) tail shortening; regulation of translational initiation; translational initiation |
| UniProt Code: | P23588 |
| NCBI GenInfo Identifier: | 205371761 |
| NCBI Gene ID: | 1975 |
| NCBI Accession: | P23588.2 |
| UniProt Secondary Accession: | P23588,Q4G0E3, Q53HQ2, Q6GPH5, Q6IB46, Q8WYK5, B4DS13 |
| UniProt Related Accession: | P23588 |
| Molecular Weight: | 64,805 Da |
| NCBI Full Name: | Eukaryotic translation initiation factor 4B |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 4B |
| NCBI Official Symbol: | EIF4B |
| NCBI Official Synonym Symbols: | EIF-4B; PRO1843 |
| NCBI Protein Information: | eukaryotic translation initiation factor 4B |
| UniProt Protein Name: | Eukaryotic translation initiation factor 4B |
| Protein Family: | Eukaryotic translation initiation factor |
| UniProt Gene Name: | EIF4B |
| UniProt Entry Name: | IF4B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EIF4B antibody (CAB13300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of U2OS cells using EIF4B antibody (CAB13300). Blue: DAPI for nuclear staining. |
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