Immunology Antibodies 2
Anti-EIF4A2 Antibody (CAB3849)
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- SKU:
- CAB3849
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-EIF4A2 Antibody |
| Antibody SKU: | CAB3849 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EIF4A2 (NP_001958.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, U-251MG, Raji, HepG2, HT-1080, Mouse liver, Mouse brain, Mouse spleen, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EIF4A2 (NP_001958.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSGG SADY NREH GGPE GMDP DGVI ESNW NEIV DNFD DMNL KESL LRGI YAYG FEKP SAIQ QRAI IPCI KGYD VIAQ AQSG TGKT ATFA ISIL QQLE IEFK ETQA LVLA PTRE LAQQ IQKV ILAL GDYM GATC HACI GGTN VRNE MQKL QAEA PHIV VGTP GRVF DMLN RRYL SPKW IKMF VLDE ADEM LSRG FKDQ IYEI FQKL NTSI QVVL LSAT MPTD VLEV TKKF MRDP IRIL VKKE ELTL EGIK QFYI NVER EEWK LDTL CDLY ET |
| Gene ID: | 1974 |
| Uniprot: | Q14240 |
| Cellular Location: | |
| Calculated MW: | 46kDa |
| Observed MW: | 46kDa |
| Synonyms: | EIF4A2, BM-010, DDX2B, EIF4A, EIF4F, eIF-4A-II, eIF4A-II |
| Background: |
| UniProt Protein Function: | EIF4A2: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. Belongs to the DEAD box helicase family. eIF4A subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.6.4.13; Helicase; RNA-binding; Translation; Translation initiation Chromosomal Location of Human Ortholog: 3q27.3 Cellular Component: cytosol; eukaryotic translation initiation factor 4F complex; perinuclear region of cytoplasm Molecular Function:ATP-dependent RNA helicase activity; ATPase activity; helicase activity; protein binding Biological Process: poly(A) tail shortening; RNA secondary structure unwinding; translational initiation |
| UniProt Code: | Q14240 |
| NCBI GenInfo Identifier: | 45645183 |
| NCBI Gene ID: | 1974 |
| NCBI Accession: | Q14240.2 |
| UniProt Secondary Accession: | Q14240,Q53XJ6, Q96B90, Q96EA8, D3DNU9, |
| UniProt Related Accession: | Q14240 |
| Molecular Weight: | |
| NCBI Full Name: | Eukaryotic initiation factor 4A-II |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 4A2 |
| NCBI Official Symbol: | EIF4A2 |
| NCBI Official Synonym Symbols: | DDX2B; EIF4A; EIF4F; BM-010; eIF4A-II; eIF-4A-II |
| NCBI Protein Information: | eukaryotic initiation factor 4A-II |
| UniProt Protein Name: | Eukaryotic initiation factor 4A-II |
| UniProt Synonym Protein Names: | ATP-dependent RNA helicase eIF4A-2 |
| Protein Family: | Eukaryotic initiation factor |
| UniProt Gene Name: | EIF4A2 |
 | Western blot analysis of extracts of various cell lines, using EIF4A2 antibody (CAB3849) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat brain using EIF4A2 antibody (CAB3849) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat heart using EIF4A2 antibody (CAB3849) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using EIF4A2 antibody (CAB3849) at dilution of 1:100 (40x lens). |
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