Metabolism Antibodies 3
Anti-EIF3K Antibody (CAB9969)
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- SKU:
- CAB9969
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-EIF3K Antibody |
| Antibody SKU: | CAB9969 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human EIF3K (NP_037366.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, 293T, SGC-7901, HeLa, THP-1, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human EIF3K (NP_037366.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAMF EQMR ANVG KLLK GIDR YNPE NLAT LERY VETQ AKEN AYDL EANL AVLK LYQF NPAF FQTT VTAQ ILLK ALTN LPHT DFTL CKCM IDQA HQEE RPIR QILY LGDL LETC HFQA FWQA LDEN MDLL EGIT GFED SVRK FICH VVGI TYQH IDRW LLAE MLGD LSDS QLKV WMSK YGWS ADES GQIF ICSQ EESI KPKN IVEK IDFD SVSS IMAS SQ |
| Gene ID: | 27335 |
| Uniprot: | Q9UBQ5 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 24kDa/25kDa |
| Observed MW: | 25kDa |
| Synonyms: | EIF3K, ARG134, EIF3-p28, EIF3S12, HSPC029, M9, MSTP001, PLAC-24, PLAC24, PRO1474, PTD001 |
| Background: |
| UniProt Protein Function: | EIF3K: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Belongs to the eIF-3 subunit K family. |
| UniProt Protein Details: | Protein type:Translation initiation; Translation Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: cytoplasm; cytosol; eukaryotic translation initiation factor 3 complex; membrane Molecular Function:protein binding; translation initiation factor activity Biological Process: translational initiation |
| NCBI Summary: | The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9UBQ5 |
| NCBI GenInfo Identifier: | 23396628 |
| NCBI Gene ID: | 27335 |
| NCBI Accession: | Q9UBQ5.1 |
| UniProt Secondary Accession: | Q9UBQ5,Q96IQ0, Q9Y6D1, A8K0I9, B7ZAM9, |
| UniProt Related Accession: | Q9UBQ5 |
| Molecular Weight: | 24,484 Da |
| NCBI Full Name: | Eukaryotic translation initiation factor 3 subunit K |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 3 subunit K |
| NCBI Official Symbol: | EIF3K |
| NCBI Official Synonym Symbols: | M9; ARG134; PLAC24; PTD001; EIF3S12; HSPC029; MSTP001; PLAC-24; PRO1474; EIF3-p28 |
| NCBI Protein Information: | eukaryotic translation initiation factor 3 subunit K |
| UniProt Protein Name: | Eukaryotic translation initiation factor 3 subunit K |
| UniProt Synonym Protein Names: | Eukaryotic translation initiation factor 3 subunit 12 |
| UniProt Gene Name: | EIF3K |
| UniProt Entry Name: | EIF3K_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EIF3K antibody (CAB9969) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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