Metabolism Antibodies 3
Anti-EIF3J Antibody (CAB8637)
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- SKU:
- CAB8637
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-EIF3J Antibody |
| Antibody SKU: | CAB8637 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human EIF3J (NP_003749.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SKOV3, HeLa, Jurkat, A-549, U-87MG, LO2, Mouse testis, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human EIF3J (NP_003749.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAA AAAA GDSD SWDA DAFS VEDP VRKV GGGG TAGG DRWE GEDE DEDV KDNW DDDD DEKK EEAE VKPE VKIS EKKK IAEK IKEK ERQQ KKRQ EEIK KRLE EPEE PKVL TPEE QLAD KLRL KKLQ EESD LELA KETF GVNN AVYG IDAM NPSS RDDF TEFG KLLK DKIT QYEK SLYY ASFL EVLV RDVC ISLE IDDL KKIT NSLT VLCS EKQK QEKQ SKAK KKKK GVVP GGGL KATM KDDL ADYG GYDG GYVQ DYED FM |
| Gene ID: | 8669 |
| Uniprot: | O75822 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 22kDa/23kDa/29kDa |
| Observed MW: | 37kDa |
| Synonyms: | EIF3J, EIF3S1, eIF3-alpha, eIF3-p35 |
| Background: | This gene encodes a core subunit of the eukaryotic initiation factor 3 complex, which participates in the initiation of translation by aiding in the recruitment of protein and mRNA components to the 40S ribosome. There are pseudogenes for this gene on chromosomes 1, 3, and 9. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | eIF3-alpha: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Belongs to the eIF-3 subunit J family. |
| UniProt Protein Details: | Protein type:Translation initiation; Translation Chromosomal Location of Human Ortholog: 15q21.1 Cellular Component: cytosol; eukaryotic translation initiation factor 3 complex Molecular Function:protein binding; translation initiation factor activity Biological Process: cellular protein metabolic process; formation of translation preinitiation complex; gene expression; regulation of translational initiation; translation; translational initiation |
| NCBI Summary: | This gene encodes a core subunit of the eukaryotic initiation factor 3 complex, which participates in the initiation of translation by aiding in the recruitment of protein and mRNA components to the 40S ribosome. There are pseudogenes for this gene on chromosomes 1, 3, and 9. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2013] |
| UniProt Code: | O75822 |
| NCBI GenInfo Identifier: | 23503065 |
| NCBI Gene ID: | 8669 |
| NCBI Accession: | O75822.2 |
| UniProt Secondary Accession: | O75822,Q9BUD2, Q9H8Q2, Q9UI65, B4DUI3, F5H425, |
| UniProt Related Accession: | O75822 |
| Molecular Weight: | 23,027 Da |
| NCBI Full Name: | Eukaryotic translation initiation factor 3 subunit J |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 3 subunit J |
| NCBI Official Symbol: | EIF3J |
| NCBI Official Synonym Symbols: | EIF3S1; eIF3-p35; eIF3-alpha |
| NCBI Protein Information: | eukaryotic translation initiation factor 3 subunit J |
| UniProt Protein Name: | Eukaryotic translation initiation factor 3 subunit J |
| UniProt Synonym Protein Names: | Eukaryotic translation initiation factor 3 subunit 1 |
| UniProt Gene Name: | EIF3J |
| UniProt Entry Name: | EIF3J_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EIF3J antibody (CAB8637) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat brain using EIF3J antibody (CAB8637) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung using EIF3J antibody (CAB8637) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using EIF3J antibody (CAB8637) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using EIF3J antibody (CAB8637) at dilution of 1:100 (40x lens). |
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