Metabolism Antibodies 2
Anti-EIF3I Antibody (CAB6582)
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- SKU:
- CAB6582
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-EIF3I Antibody |
| Antibody SKU: | CAB6582 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human EIF3I (NP_003748.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | NCI-H460, LO2, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human EIF3I (NP_003748.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKPI LLQG HERS ITQI KYNR EGDL LFTV AKDP IVNV WYSV NGER LGTY MGHT GAVW CVDA DWDT KHVL TGSA DNSC RLWD CETG KQLA LLKT NSAV RTCG FDFG GNII MFST DKQM GYQC FVSF FDLR DPSQ IDNN EPYM KIPC NDSK ITSA VWGP LGEC IIAG HESG ELNQ YSAK SGEV LVNV KEHS RQIN DIQL SRDM TMFV TASK DNTA KLFD STTL EHQK TFRT ERPV NSAA LSPN YDHV VLGG GQEA MDVT TTST RIGK FEAR FFHL AFEE EFGR VKGH FGPI NSVA FHPD GKSY SSGG EDGY VRIH YFDP QYFE FEFE A |
| Gene ID: | 8668 |
| Uniprot: | Q13347 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 36kDa |
| Observed MW: | 37kDa |
| Synonyms: | EIF3I, EIF3S2, PRO2242, TRIP-1, TRIP1, eIF3-beta, eIF3-p36 |
| Background: |
| UniProt Protein Function: | eIF3-beta: eukaryotic translation initiation factor 3 subunit 2. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. eIF-3 is composed of at least 12 different subunits. Phosphorylated by TGF-beta type II receptor. |
| UniProt Protein Details: | Protein type:Translation; Translation initiation Chromosomal Location of Human Ortholog: 1p34.1 Cellular Component: eukaryotic translation initiation factor 3 complex; cytosol Molecular Function:protein binding; translation initiation factor activity Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression; formation of translation preinitiation complex; regulation of translational initiation |
| UniProt Code: | Q13347 |
| NCBI GenInfo Identifier: | 2494895 |
| NCBI Gene ID: | 8668 |
| NCBI Accession: | Q13347.1 |
| UniProt Related Accession: | Q13347 |
| Molecular Weight: | 325 |
| NCBI Full Name: | Eukaryotic translation initiation factor 3 subunit I |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 3, subunit I |
| NCBI Official Symbol: | EIF3I |
| NCBI Official Synonym Symbols: | TRIP1; EIF3S2; TRIP-1; PRO2242; eIF3-p36; eIF3-beta |
| NCBI Protein Information: | eukaryotic translation initiation factor 3 subunit I; EIF3I; eIF3 p36; eIF-3-beta; predicted protein of HQ2242; TGFbeta receptor-interacting protein 1; TGF-beta receptor-interacting protein 1; eukaryotic translation initiation factor 3 subunit 2; eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa |
| UniProt Protein Name: | Eukaryotic translation initiation factor 3 subunit I |
| UniProt Synonym Protein Names: | Eukaryotic translation initiation factor 3 subunit 2; TGF-beta receptor-interacting protein 1; eIF3 p36 |
| UniProt Gene Name: | EIF3I |
| UniProt Entry Name: | EIF3I_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EIF3I antibody (CAB6582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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