Name: Anti-EIF3H Antibody (CAB18026)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB18026
Availability: OutOfStock

Antibody Name:	Anti-EIF3H Antibody [KO Validated]
Antibody SKU:	CAB18026
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EIF3H (NP_003747.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EIF3H (NP_003747.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NCFP FPQH TEDD ADFD EVQY QMEM MRSL RHVN IDHL HVGW YQST YYGS FVTR ALLD SQFS YQHA IEES VVLI YDPI KTAQ GSLS LKAY RLTP KLME VCKE KDFS PEAL KKAN ITFE YMFE EVPI VIKN SHLI NVLM WELE KKSA VADK HELL SLAS SNHL GKNL QLLM DRVD EMSQ DIVK YNTY MRNT SKQQ QQKH QYQQ RRQQ ENMQ RQSR GEPP LPEE DLSK LFKP PQPP ARMD SLLI AGQI NTYC QNIK EFTA QNLG K
Gene ID:	8667
Uniprot:	O15372
Cellular Location:	Cytoplasm
Calculated MW:	39kDa
Observed MW:	40kDa

Anti-EIF3H Antibody [KO Validated] (CAB18026)

Western blot analysis of extracts from normal (control) and EIF3H knockout (KO) HeLa cells, using EIF3H antibody (CAB18026) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Synonyms:	EIF3H, EIF3S3, eIF3-gamma, eIF3-p40
Background:

UniProt Protein Function:	eIF3S3: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with RNF139; the interaction leads to protein translation inhibitions in a ubiquitination- dependent manner. Belongs to the eIF-3 subunit H family.
UniProt Protein Details:	Protein type:Translation; Translation initiation Chromosomal Location of Human Ortholog: 8q24.11 Cellular Component: eukaryotic translation initiation factor 3 complex; membrane; cytosol Molecular Function:protein binding; translation initiation factor activity Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression; formation of translation preinitiation complex; regulation of translational initiation
UniProt Code:	O15372
NCBI GenInfo Identifier:	6685512
NCBI Gene ID:	8667
NCBI Accession:	O15372.1
UniProt Related Accession:	O15372
Molecular Weight:	352
NCBI Full Name:	Eukaryotic translation initiation factor 3 subunit H
NCBI Synonym Full Names:	eukaryotic translation initiation factor 3, subunit H
NCBI Official Symbol:	EIF3H
NCBI Official Synonym Symbols:	EIF3S3; eIF3-p40; eIF3-gamma
NCBI Protein Information:	eukaryotic translation initiation factor 3 subunit H; EIF3H; eIF-3-gamma; eIF3 p40 subunit; eukaryotic translation initiation factor 3 subunit 3; eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD); eukaryotic translation initiation factor
UniProt Protein Name:	Eukaryotic translation initiation factor 3 subunit H
UniProt Synonym Protein Names:	Eukaryotic translation initiation factor 3 subunit 3; eIF-3-gammaeIF3 p40 subunit
UniProt Gene Name:	EIF3H
UniProt Entry Name:	EIF3H_HUMAN